LOCUS       sequence-56846-e        3269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Koreishi M, Honjo Y, Satoh A
  TITLE     Detection of in situ cleaved p115 with the cut specific antibodies 
            in rapid protein inactivation system by tobacco etch viral protease 
            cleavage
  JOURNAL   Antibody Technology Journal. 2011;1:5-11
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antibody 
            Technology Journal."; date: "2011"; volume: "1"; pages: "5-1"
COMMENT     Sequence Label: GFP-Golgin-84-TEV #709#243
FEATURES             Location/Qualifiers
     source          1..3269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(59..61,62..64,65..775)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(59..61,62..64,65..775)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(104..125)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(365..384)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     712..733
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1652..1672
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3198..3239
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gctgtagctt ggtccgagct cggatccact agtccagtgt ggtggaattg cccttaccat
       61 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      121 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      181 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      241 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      301 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      361 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      421 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      481 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      541 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      601 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      661 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      721 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
      781 ccggactcag atctcgagct caagcttcga attctcttgg tttgttgatc ttgctggaaa
      841 ggcagaagat cttttaaacc gagttgatca aggggctgca acagctctca gtaggaaaga
      901 caatgccagc aacatatata gcaaaaatac tgactatact gaacttcacc agcaaaatac
      961 agatttgata tatcagactg gacctaaatc tacgtatatt tcatcagcag ctgataacat
     1021 tcgaaatcaa aaagccacca tcttagctgg cactgcaaat gtgaaagtag gatctcggac
     1081 accagtagag gcctctcatc ctgctgaaaa tgcatctgtt cctaggcctt catcccattt
     1141 tgtgcgaaga aaaaagtcgg aacctgatga tgagctgctg tttgattttc ttaatagttc
     1201 acagaaggag cctaccggga gggtggaaat cagaaaggaa aaaggcaaga cacctgtctt
     1261 tcagagctct cagacatcaa gtgtcagttc tgtgaacccc agtgtaacca ccatcaaaac
     1321 cattgaagaa aattcttttg ggagccaaac ccacgaagct gccagtaact cagattctag
     1381 ccatgaaggt caagaggaat cttcaaagga aaatgtgtca tcaaatgctg cctgccctga
     1441 ccacacccca acacctaatg atgatggcaa atcacatgaa ctgtctaacc ttcgactgga
     1501 gaatcagctg ctgaggaatg aagttcagtc tttaaatcaa gaaatggcct cgttactcca
     1561 aagatccaaa gagactcaag aagaattaaa caaagcaaga gcaagagttg aaaagtggaa
     1621 tgctgaccat tcaaagagtg atcgaatgac tgaaaacctg tacttccagg gccgaggact
     1681 ccgagcccaa gcagatgacc tgactgaagc tgtggctgca aaggattccc agctggctgt
     1741 actgaaagtg agactccagg aagctgacca gctactgagt actcgcacag aagcattaga
     1801 agccttacag agtgaaaaat cacgaataat gcaggatcaa agtgaaggta acagcctgca
     1861 gaatcaagct ctgcagactc ttcaggagag actgcatgaa gcggatgcca ctctgaagag
     1921 agagcaggag agctataaac agatgcagag cgagtttgct gcacgcctta ataaagtgga
     1981 aatggaacgt cagaatttag cagaagcaat tacactggcc gaaagaaaat actcagatga
     2041 gaagaagagg gttgatgaac tgcagcagca agtcaagctg tataagttga acttggagtc
     2101 ctctaagcag gaattaattg actacaagca aaaagctact agaatactgc aatctaagga
     2161 aaaattgatt aacagcttga aagaaggctc tggttttgaa ggcctagata gcagcactgc
     2221 cagtagcatg gagctggaag aacttcggca tgagaaagag atgcagaggg aggaaataca
     2281 gaagctgatg ggccagatac atcagctcag atccgaatta caggatatgg aggcacagca
     2341 agttaatgaa gcagaatcag caagagaaca gttacaggat ctgcatgacc aaatagctgg
     2401 gcagaaagca tccaaacaag aactagagac agaactggag cgactgaagc aggagttcca
     2461 ctatatagaa gaagatcttt atcgaacaaa gaacacattg caaagcagaa ttaaagatcg
     2521 agacgaagaa attcaaaaac tcaggaatca gcttaccaat aaaactttaa gcaatagcag
     2581 tcagtctgag ttagaaaatc gactccatca gctaacagag actctcatcc agaaacagac
     2641 catgctggag agtctcagca cagaaaagaa ctccctggtc tttcaactgg agcgcctcga
     2701 acagcagatg aactccgcct ctggaagtag tagtaatggg tcttcgatta atatgtctgg
     2761 aattgacaat ggtgaaggca ctcgtctgcg aaatgttcct gttcttttta atgacacaga
     2821 aactaatctg gcaggaatgt acggaaaagt tcgcaaagct gctagttcaa ttgatcagtt
     2881 tagtattcgc ctgggaattt ttctccgaag ataccccata gcgcgagttt ttgtaattat
     2941 atatatggct ttgcttcacc tctgggccat gattgttctg ttgacttaca caccagaaat
     3001 gcaccacgac caaccatatg gcaaatgagg atccaccgga tctagataac tgatcataat
     3061 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3121 gaacctgaaa caagggcaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     3181 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     3241 gtaccggtca tcatcacctc accatggta
//
