LOCUS       sequence-56862-e        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Koreishi M, Yu S, Oda M, Honjo Y, Satoh A
  TITLE     CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking.
  JOURNAL   PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 
            2013 Jan 18.
  PUBMED    23349870
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e54382. doi"; pages: " 
            10.1371/journal.pone.0054382. Epub 2013 Jan 18"
COMMENT     Sequence Label: pQC-SEAP #M004#725
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             62..1564
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
ORIGIN
        1 gcggccgcga attcactagt gatttaatac gactcactat agggagaccc aagcttcctg
       61 catgctgctg ctgctgctgc tgctgggcct gaggctacag ctctccctgg gcatcatccc
      121 agttgaggag gagaacccgg acttctggaa ccgcgaggca gccgaggccc tgggtgccgc
      181 caagaagctg cagcctgcac agacagccgc caagaacctc atcatcttcc tgggcgatgg
      241 gatgggggtg tctacggtga cagctgccag gatcctaaaa gggcagaaga aggacaaact
      301 ggggcctgag atacccctgg ccatggaccg cttcccatat gtggctctgt ccaagacata
      361 caatgtagac aaacatgtgc cagacagtgg agccacagcc acggcctacc tgtgcggggt
      421 caagggcaac ttccagacca ttggcttgag tgcagccgcc cgctttaacc agtgcaacac
      481 gacacgcggc aacgaggtca tctccgtgat gaatcgggcc aagaaagcag ggaagtcagt
      541 gggagtggta accaccacac gagtgcagca cgcctcgcca gccggcacct acgcccacac
      601 ggtgaaccgc aactggtact cggacgccga cgtgcctgcc tcggcccgcc aggaggggtg
      661 ccaggacatc gctacgcagc tcatctccaa catggacatt gacgtgatcc taggtggagg
      721 ccgaaagtac atgtttcgca tgggaacccc agaccctgag tacccagatg actacagcca
      781 aggtgggacc aggctggacg ggaagaatct ggtgcaggaa tggctggcga agcgccaggg
      841 tgcccggtat gtgtggaacc gcactgagct catgcaggct tccctggacc cgtctgtgac
      901 ccatctcatg ggtctctttg agcctggaga catgaaatac gagatccacc gagactccac
      961 actggacccc tccctgatgg agatgacaga ggctgccctg cgcctgctga gcaggaaccc
     1021 ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac catggtcatc atgaaagcag
     1081 ggcttaccgg gcactgactg agacgatcat gttcgacgac gccattgaga gggcgggcca
     1141 gctcaccagc gaggaggaca cgctgagcct cgtcactgcc gaccactccc acgtcttctc
     1201 cttcggaggc taccccctgc gagggagctc catcttcggg ctggcccctg gcaaggcccg
     1261 ggacaggaag gcctacacgg tcctcctata cggaaacggt ccaggctatg tgctcaagga
     1321 cggcgcccgg ccggatgtta ccgagagcga gagcgggagc cccgagtatc ggcagcagtc
     1381 agcagtgccc ctggacgaag agacccacgc aggcgaggac gtggcggtgt tcgcgcgcgg
     1441 cccgcaggcg cacctggttc acggcgtgca ggagcagacc ttcatagcgc acgtcatggc
     1501 cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg gcgccccccg ccggcaccac
     1561 cgacgccgcg cacccgggtt aacccgtggt ccccgcgttg cttcctctgc tggccgggac
     1621 atcaggtggc ccccgctgaa ttggaatcga tccatcacac tggcggccgc
//
