LOCUS       sequence-56880-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Soriano P.
  TITLE     Generalized lacZ expression with the ROSA26 Cre reporter strain.
  JOURNAL   Nat Genet. 1999 Jan;21(1):70-1.
  PUBMED    9916792
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "1999-01"; volume: "21(1)"; pages: "70-1"
COMMENT     Sequence Label: PGKneotpAlox2
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(56..89)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    118..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    372..506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    626..760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ccctcgaggt cgacggtatc gataagctag cttgggctgc aggtcgaggg acctaataac
       61 ttcgtatagc atacattata cgaagttata ttaagggttc cggatcagct tgatggggat
      121 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      241 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      301 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
      361 ctagagtcgc agatccagac atgataagat acattgatga gtttggacaa accacaacta
      421 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      481 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      541 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      601 ctgattatga tcctctagag tcgcagatcc agacatgata agatacattg atgagtttgg
      661 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      721 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaac
//
