LOCUS       sequence-56885-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of human G9a mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Wang L, Xu S, Lee JE, Baldridge A, Grullon S, Peng W, Ge K
  TITLE     Histone H3K9 methyltransferase G9a represses PPARgamma expression 
            and adipogenesis.
  JOURNAL   EMBO J. 2012 Nov 23. doi: 10.1038/emboj.2012.306.
  PUBMED    23178591
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2012 Nov 23. doi: 10.1038/emboj.2012.306."
COMMENT     Sequence Label: MSCVhygro-F-G9adSET
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatcctg gccaccatgg actacaagga
       61 cgacgatgac aagatggcgg cggcggcggg agctgcagcg gcggcggccg ccgaggggga
      121 ggcccccgct gagatggggg cgctgctgct ggagaaggaa accagaggag ccaccgagag
      181 agttcatggc tctttggggg acacccctcg tagtgaagaa accctgccca aggccaaccc
      241 cgactccctg gagcctgctg gcccctcatc tccagcctct gtcactgtca ctgttggtga
      301 tgagggggct gacacccctg taggggctac accactcatt ggggatgaat ctgagaatct
      361 tgagggagat ggggacctcc gtgggggccg catcctgctg ggccatgcca caaagtcatt
      421 cccctcttcc cccagcaagg ggggttcctg ccctagccgg gccaagatgt caatgacagg
      481 ggcgggaaaa tcacctccat ctgtccagag tttggctatg aggctactga gtatgccagg
      541 agcccaggga gctgcagcag cagggtctga accccctcca gccaccacga gcccagaggg
      601 acagcccaag gtccaccgag cccgcaaaac catgtccaaa ccaggaaatg gacagccccc
      661 ggtccctgag aagcggcccc ctgaaataca gcatttccgc atgagtgatg atgtccactc
      721 actgggaaag gtgacctcag atctggccaa aaggaggaag ctgaactcag gaggtggcct
      781 gtcggaggag ttaggttctg cccggcgttc aggagaagtg accctgacga aaggggaccc
      841 cgggtccctg gaggagtggg agacggtggt gggtgatgac ttcagtctct actatgattc
      901 ctactctgtg gatgagcgcg tggactc
//
