LOCUS       sequence-56886-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet16
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gcctaggaca gtacaaactt atcctgaacg
      121 gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc gcggaaaaag
      181 ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac gacgacgcta
      241 ccaaaaccta caccgtaacg gaaggatcct gtttaagcta tgaaacggaa atattgacag
      301 tagaatatgg attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt
      361 atagcgttga taataatgga aatatttata cacaacctgt agcacaatgg cacgatcgcg
      421 gagaacaaga ggtgtttgag tattgtttgg aagatggttc attgattcgg gcaacaaaag
      481 accataagtt tatgactgtt gatggtcaaa tgttgccaat tgatgaaata tttgaacgtg
      541 aattggattt gatgcgggtt gataatttgc cgaatatcaa aatagccaca cgtaaatatt
      601 taggcaaaca aaatgtctat gacattggag ttgagcgcga ccataatttt gcactcaaaa
      661 atggcttcat agcttctaat tgtttcaatg gtacccagta caaacttatc ctgaacggta
      721 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
      781 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
      841 aaacctacac cgta
//
