LOCUS       sequence-56887-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet16
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagcc
       61 gccaaaacag ccaagcttat tccgttacgg tgtaggtttt ggtagcgtcg tcgtaggtcc
      121 attcaccgtc aacaccgttg tcgttagcgt actgtttgaa aactttttcc gcggtagcag
      181 cgtcaacagc ttcggtggtg gtttcacctt tcagggtttt accgttcagg ataagtttgt
      241 actgggtacc attgaaacaa ttagaagcta tgaagccatt tttgagtgca aaattatggt
      301 cgcgctcaac tccaatgtca tagacatttt gtttgcctaa atatttacgt gtggctattt
      361 tgatattcgg caaattatca acccgcatca aatccaattc acgttcaaat atttcatcaa
      421 ttggcaacat ttgaccatca acagtcataa acttatggtc ttttgttgcc cgaatcaatg
      481 aaccatcttc caaacaatac tcaaacacct cttgttctcc gcgatcgtgc cattgtgcta
      541 caggttgtgt ataaatattt ccattattat caacgctata aacagtacat tcgatgcgct
      601 tttctacaat tttaccaatc ggtaataatc catattctac tgtcaatatt tccgtttcat
      661 agcttaaaca ggatccttcc gttacggtgt aggttttggt agcgtcgtcg taggtccatt
      721 caccgtcaac accgttgtcg ttagcgtact gtttgaaaac tttttccgcg gtagcagcgt
      781 caacagcttc ggtggtggtt tcacctttca gggttttacc gttcaggata agtttgtact
      841 gtcctaggct
//
