LOCUS       sequence-56893-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, 
            Kinzler KW
  TITLE     Activation of beta-catenin-Tcf signaling in colon cancer by 
            mutations in beta-catenin or APC.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1787-90.
  PUBMED    9065402
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1997 Mar 21. 275(5307):1787-90."
COMMENT     Sequence Label: pCI-neo beta catenin S33Y
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          126..258
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     241..260
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        303..321
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             378..413
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagt gcttctgaca caacagtctc
       61 gaacttaagc tgcagtgact ctcttaaggt agccttgcag aagttggtcg tgaggcactg
      121 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
      181 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
      241 ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta
      301 cttaatacga ctcactatag gctagaacca ccatggctac tcaagctgat ttgatggagt
      361 tggacatggc catggaacca gacagaaaag cggctgttag tcactggcag caacagtctt
      421 acctggacta tggaatccat tctggtgcca ctaccacagc tccttctctg agtggtaaag
      481 gcaatcctga ggaagaggat gtggatacct cccaagtcct gtatgagtgg gaacagggat
      541 tttctcagtc cttcactcaa gaacaagtag ctgatattga tggacagtat gcaatgactc
      601 gagctcagag ggtacgagct gctatgttcc ctgagacatt agatgagggc atgcagatcc
      661 catctacaca gtttgatgct gctcatccca ctaatgtcca gcgtttggct gaaccatcac
      721 agatgctgaa acatgcagtt gtaaacttga ttaactatca agatgatgca gaacttgcca
      781 cacgtgcaat ccctgaactg acaaaactgc taaatgacga ggaccaggtg gtggttaata
      841 aggctgcagt tatggtccat cagctttcta aaaaggaagc ttccagacac gctatcatgc
      901 gttctcctca gatggtgtct gctattgtac gtaccatgc
//
