LOCUS       sequence-56896-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet20
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccta ggacagtaca aacttatcct gaacggtaaa
      121 accctgaaag gtgaaaccac caccgaagct gttgacgctg ctaccgcgga aaaagttttc
      181 aaacagtacg ctaacgacaa cggtgttgac ggtgaatgga cctacgacga cgctaccaaa
      241 acctacaccg taacggaagg atcctatagt gttgattaca acgaaccaat aatcattaaa
      301 gagaatggag aaataaaagt tgttaaaatt ggagaactta tagataaaat tattgaaaac
      361 tcagagaata ttagaagaga gggcatctta gagatagcaa aatgtaaagg tattgaagtt
      421 attgccttta acagcaatta caaatttaaa ttcatgcctg tttcggaggt ttcaaggcat
      481 ccagttagtg agatgtttga aatagttgtt gaagggaata aaaaggttag agttaccaga
      541 agccatagtg tctttaccat aagagataat gaggtagttc caataagagt tgatgagcta
      601 aaagttggag atatattagt tttagcaaaa gaattgccga atattgaaga agatattgaa
      661 atagataaaa aatttagtaa aatattgggt tacataattg cggaaggtta ttatgatgac
      721 aaaaaaattg tattatctta t
//
