LOCUS       sequence-56899-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet20
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gccgccaaaa cagccaagct tattccgtta cggtgtaggt tttggtagcg
      121 tcgtcgtagg tccattcacc gtcaacaccg ttgtcgttag cgtactgttt gaaaactttt
      181 tccgcggtag cagcgtcaac agcttcggtg gtggtttcac ctttcagggt tttaccgttc
      241 aggataagtt tgtactgggt accgtggatg gtgttgtgta atacaaatcc tccaaatcca
      301 gcaacgaagt tttctgcatt tggaacagtt aaatcatagg catatccact ggttggctct
      361 accttattaa tctctttaat ttttaagaat atgaaatcag agtttataag tttttcgagt
      421 tttctattgg tatatatatt tgttattctc cttacctcat aaacttcaat atcatttata
      481 ctctttttat taatggtggc gttttctttt atttgtaaaa gtaagaatat aacatcttca
      541 gccaactcct ttgagacaaa tacttttgca tctttaccat taaatatccc atctatgaat
      601 gattttttta tttcataagg agatttaaag attatagagg gaattctttt attcttaact
      661 ctcaattttt ttagtaaatt gataattttt ttattcttta cttcaatttg aatatttaaa
      721 tctttactat agatggttat atccgaattc aaagatttga aataatcaat tgtttcattt
      781 ataaactctt tttcattgta atcataagat aatacaattt ttttgtcatc ataataacct
      841 tccgcaatta tgtaacccaa tattttact
//
