LOCUS       sequence-56900-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet21
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatattttgt ttaactttaa taaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag cctaggacag tacaaactta tcctgaacgg
      121 taaaaccctg aaaggtgaaa ccaccaccga agctgttgac gctgctaccg cggaaaaagt
      181 tttcaaacag tacgctaacg acaacggtgt tgacggtgaa tggacctacg acgacgctac
      241 caaaacctac accgtaacgg aaggatccaa gtgcgttgac tacgatacag aagtcctact
      301 tggagatggg agaaagagga agataggaga gatcgttgag gaagcgataa agaaagcaga
      361 aaaagaggga aaacttggaa gggttgacga tggcttctac gctccaataa acctggagct
      421 gtacgcttta gatgtgagaa cccttaaggt caggaaggtc aaagctgaca tagcgtggaa
      481 gagaacaacc ccggagaaga tgttgaggat tagaactaag aggggtaggg aaattagggt
      541 aaccccaacg cacccattct tcacgttaga ggagggaagg ataaagacga agaaagctta
      601 tgagctaaag gttggggaga agatagccac ccccagggag gaagcacccg aagctgagat
      661 attttgggat gaagttgttg agattgagga gtacaagccc aataactctt gggtttacga
      721 tctccaagtt ccagagcacc acaacttcat agcgaacgga atattcgtgc acaatagcca
      781 actcggtacc cagtacaaac ttatcctgaa cggtaaaacc ctgaaaggtg aaaccaccac
      841 cgaagctgtt gacgctgcta c
//
