LOCUS       sequence-56902-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet22
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..86
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tttgtttaac tttaataagg agatatacca tgggcagcag ccatcatcat catcatcaca
       61 gcagcggcct ggtgccgcgc ggcagcctag gacagtacaa acttatcctg aacggtaaaa
      121 ccctgaaagg tgaaaccacc accgaagctg ttgacgctgc taccgcggaa aaagttttca
      181 aacagtacgc taacgacaac ggtgttgacg gtgaatggac ctacgacgac gctaccaaaa
      241 cctacaccgt aacggaagga tccctctgcg tggccccgga taccttgata aataccgaca
      301 acggaagggt agagatcggg aagttcgtgg aggagtggat gaaggaagtt ggagagataa
      361 gcgaggaagg gatcagctat gcaccatgct tcaggaaggt cgagacgttc aaagacggga
      421 agatagttga atcaccaatt agaagggttt ggaaactgag agctccgaag aagctcgtga
      481 ggataaaaac ggagaatgga aggagcatag ccctaaccag ggagaccaag ttactaacga
      541 taaatgatgg agaattatcc tgggttgagg ccggggaggt taaggtagga acatacgtgg
      601 gaacggtaaa aagtgaaaaa gacgtgattc caggggctgg aaagacaata agggatgttt
      661 caaaactcta caatatggaa atggaagtta aggattactt aaccagggaa gaagtgagaa
      721 aagccattga aaagcttgaa gaaattatga atccaatgaa tatcaagatt cctggggtgc
      781 aagaaagtta cgaagagctg ttaaggaagc ttgaaactac caacgacgag agggttagga
      841 acgagacatt gatcctccta agtgacgtta gcgatgccca c
//
