LOCUS       sequence-56904-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet23
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tattttgttt aactttaata aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc taggacagta caaacttatc ctgaacggta
      121 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
      181 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
      241 aaacctacac cgtaacggaa ggatccaatt gcttcccggg agatacaaga atattagttc
      301 aaattaatgg aactccgcag agagttacac ttaaggaatt atatgagctc tttgacgagg
      361 aacattatga aagcatggtg tacgtaagga aaaagccaaa ggtagacatt aaggtatact
      421 ccttcaaccc tgaggaaggt aaggtagttc tgaccgatat tgaggaggta ataaaagccc
      481 ctgctactga tcatttaatt cgctttgaac ttgagctcgg aagtagcttt gagacaaccg
      541 tggatcaccc agtcctcgta tatgaaaatg gaaagttcgt ggagaaaagg gcatttgagg
      601 ttagggaggg gaatataata attataatcg atgaatcaac tttggaaccc cttaaggttg
      661 ctgttaaaaa aatagagttc atagaaccgc ctgaggactt tgtgttctct cttaatgcta
      721 aaaaatatca tactgtaata attaatgaaa atattgtgac gcatcagtgc gatggtaccc
      781 agtacaaact tatcctgaac ggtaaaaccc tgaaaggtga a
//
