LOCUS       sequence-56906-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Gueguen E, Cascales E
  TITLE     Promoter swapping unveils the role of the Citrobacter rodentium CTS1
            type VI secretion system in interbacterial competition.
  JOURNAL   Appl Environ Microbiol. 2013 Jan;79(1):32-8. doi: 
            10.1128/AEM.02504-12. Epub 2012 Oct 12.
  PUBMED    23064344
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02504-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-01"; volume: "79"; issue: "1"; pages: "32-8"
COMMENT     Sequence Label: pRL128
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    101..148
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(386..391,392..407,408..414)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    422..438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     427..449
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      complement(516..543)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(635..721)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctaata aggggatctt gaagttccta ttccgaagtt
      121 cctattctct agaaagtata ggaacttcga agcagctcca gcctacacct cactcattag
      181 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
      241 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
      301 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
      361 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
      421 attgtgagcg gataacaatt tcacacagga aacagccaaa tcgctcaaga cgtgtaatgc
      481 tgcaatctgc atgcaagctt ggcactggcc acgcaaaaag gccatccgtc aggatggcct
      541 tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
      601 gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
      661 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      721 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc gggggccatc
      781 gatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      901 tgaaaa
//
