LOCUS       sequence-56924-e        9793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 9793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH034
FEATURES             Location/Qualifiers
     source          1..9793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1399..2589
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1751..1770)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2784..2790,2791..2808,2809..2814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2829..2850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(3070..3729)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3644..3663
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3730..3832)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4690..5304
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4781..5312
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5380..5599
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             5690..6445
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    6448..6698
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7024..8199
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             8199..9170
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    9243..9716
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactcca tggtcgctgt cgagatatga cggtgttcac aaagttcctt
      781 aaattttact tttggttaca tattttttct ttttgaaacc aaatctttat ctttgtagca
      841 ctttcacggt agcgaaacgt tagtttgaat ggaaagatgc ctgcagacac ataaagacac
      901 caaactctca tcaatagttc cgtaaatttt tattgacaga acttattgac ggcagtggca
      961 ggtgtcataa aaaaaaccat gagggtaata aataatggca acagttaacc agctggtacg
     1021 caaaccacgt gctcgcaaag ttgcgaaaag caacgtgcct gcgctggaag catgcccgca
     1081 aaaacgtggc gtatgtactc gtgtatatac taccactcct aaaaaaccga actccgcgct
     1141 gcgtaaagta tgccgtgttc gtctgactaa cggtttcgaa gtgacttcct acatcggtgg
     1201 tgaaggtcac aacctgcagg agcactccgt gatcctgatc cgtggcggtc gtgttaaaga
     1261 cctcccgggt gttcgttacc acaccgtacg tggtgcgctt gactgctccg gcgttaaaga
     1321 ccgtaagcag gctcgttcca agtatggcgt gaagcgtcct aaggcttaat ggttctccaa
     1381 cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc
     1441 accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat
     1501 atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg
     1561 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
     1621 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
     1681 gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     1741 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     1801 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     1861 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     1921 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg
     1981 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     2041 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     2101 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     2161 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     2221 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     2281 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     2341 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     2401 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     2461 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     2521 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     2581 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
     2641 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
     2701 tcgcgtccgc catctcgacc aattctcatg tttgacagct tatcatccca tgggctgagg
     2761 ggctggccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2821 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2881 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgaacc ccttgcggcc
     2941 gcccgggccg tcgaccaatt ctcatgtttg acagcttatc atcgaatttc tgccattcat
     3001 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     3061 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     3121 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     3181 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     3241 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     3301 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3361 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     3421 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3481 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     3541 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3601 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3661 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3721 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     3781 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     3841 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     3901 ttctgcgaag tgatcttccg tcacaggtat ttattcgcga taagctcatg gagcggcgta
     3961 accgtcgcac aggaaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
     4021 ggacctggat tggggaggcg gttgccgccg ctgctgctga cggtgtgacg ttctctgttc
     4081 cggtcacacc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
     4141 cgctgaaagt tctgcaaagc ctgatgggac ataagtccat cagttcaacg gaagtctaca
     4201 cgaaggtttt tgcgctggat gtggctgccc ggcaccgggt gcagtttgcg atgccggagt
     4261 ctgatgcggt tgcgatgctg aaacaattat cctgagaata aatgccttgg cctttatatg
     4321 gaaatgtgga actgagtgga tatgctgttt ttgtctgtta aacagagaag ctggctgtta
     4381 tccactgaga agcgaacgaa acagtcggga aaatctccca ttatcgtaga gatccgcatt
     4441 attaatctca ggagcctgtg tagcgtttat aggaagtagt gttctgtcat gatgcctgca
     4501 agcggtaacg aaaacgattt gaatatgcct tcaggaacaa tagaaatctt cgtgcggtgt
     4561 tacgttgaag tggagcggat tatgtcagca atggacagaa caacctaatg aacacagaac
     4621 catgatgtgg tctgtccttt tacagccagt agtgctcgcc gcagtcgagc gacagggcga
     4681 agccctcggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     4741 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc ggccgccggc gttgtggata
     4801 cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc
     4861 cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg
     4921 gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat
     4981 gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac
     5041 tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc
     5101 gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt
     5161 ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat
     5221 aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg
     5281 tgccccccct tctcgaaccc tcccggtcga gtgagcgagg aagcaccagg gaacagcact
     5341 tatatattct gcttacacac gatgcctgaa aaaacttccc ttggggttat ccacttatcc
     5401 acggggatat ttttataatt atttttttta tagtttttag atcttctttt ttagagcgcc
     5461 ttgtaggcct ttatccatgc tggttctaga gaaggtgttg tgacaaattg ccctttcagt
     5521 gtgacaaatc accctcaaat gacagtcctg tctgtgacaa attgccctta accctgtgac
     5581 aaattgccct cagaagaagc tgttttttca caaagttatc cctgcttatt gactcttttt
     5641 tatttagtgt gacaatctaa aaacttgtca cacttcacat ggatctgtca tggcggaaac
     5701 agcggttatc aatcacaaga aacgtaaaaa tagcccgcga atcgtccagt caaacgacct
     5761 cactgaggcg gcatatagtc tctcccggga tcaaaaacgt atgctgtatc tgttcgttga
     5821 ccagatcaga aaatctgatg gcaccctaca ggaacatgac ggtatctgcg agatccatgt
     5881 tgctaaatat gctgaaatat tcggattgac ctctgcggaa gccagtaagg atatacggca
     5941 ggcattgaag agtttcgcgg ggaaggaagt ggttttttat cgccctgaag aggatgccgg
     6001 cgatgaaaaa ggctatgaat cttttccttg gtttatcaaa cgtgcgcaca gtccatccag
     6061 agggctttac agtgtacata tcaacccata tctcattccc ttctttatcg ggttacagaa
     6121 ccggtttacg cagtttcggc ttagtgaaac aaaagaaatc accaatccgt atgccatgcg
     6181 tttatacgaa tccctgtgtc agtatcgtaa gccggatggc tcaggcatcg tctctctgaa
     6241 aatcgactgg atcatagagc gttaccagct gcctcaaagt taccagcgta tgcctgactt
     6301 ccgccgccgc ttcctgcagg tctgtgttaa tgagatcaac agcagaactc caatgcgcct
     6361 ctcatacatt gagaaaaaga aaggccgcca gacgactcat atcgtatttt ccttccgcga
     6421 tatcacttcc atgacgacag gatagtctga gggttatctg tcacagattt gagggtggtt
     6481 cgtcacattt gttctgacct actgagggta atttgtcaca gttttgctgt ttccttcagc
     6541 ctgcatggat tttctcatac tttttgaact gtaattttta aggaagccaa atttgagggc
     6601 agtttgtcac agttgatttc cttctctttc ccttcgtcat gtgacctgat atcgggggtt
     6661 agttcgtcat cattgatgag ggttgattat cacagtttat tactctgaat tggctatccg
     6721 cgtgtgtacc tctacctgga gtttttccca cggtggatat ttcttcttgc gctgagcgta
     6781 agagctatct gacagaacag ttcttctttg cttcctcgcc agttcgctcg ctatgctcgg
     6841 ttacacggct gcggcgagcg ctagtgataa taagtgactg aggtatgtgc tcttcttatc
     6901 tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg actttgcgat
     6961 tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaacgcaaag caatgattaa
     7021 aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc tggtcatgaa
     7081 atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc gaggaaaata
     7141 acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc tcaggctatc
     7201 agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg aggacgggtt
     7261 gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt gtttggtacg
     7321 cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc ccataaaggt
     7381 ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct gaaggggcta
     7441 cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta tcacggatgg
     7501 gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct tggggaaaag
     7561 gacgatgtca cttatgcaat aaagcccact tgctggccgg ggcttgacat tattccttcc
     7621 tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga aggtaaactg
     7681 cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca tgactatgat
     7741 gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt cgtatgtgct
     7801 gctgatgtgc tgattgttcc cacgcctgct gagttgtttg actacacctc cgcactgcag
     7861 tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt cgagcctgat
     7921 gtacgtattt tgcttaccaa atacagcaat agtaatggct ctcagtcccc gtggatggag
     7981 gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg tgaaacggat
     8041 gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat tgatcaacgc
     8101 tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg caatgaaatt
     8161 ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg cctgttattc
     8221 caaaacatac gctcaatact caaccggttg aagatacttc gttatcgaca ccagctgccc
     8281 cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat gccattactt
     8341 tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt gatagtgttg
     8401 agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt actgaggacg
     8461 cactggatga tctcatccct tcttttctac tgactggtca acagacaccg gcgttcggtc
     8521 gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa gctgctgcac
     8581 ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag atggctgcat
     8641 tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt cagcgttatg
     8701 caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat gcggaaaata
     8761 tttcacgtaa gattattacc cgctgtatca acaccgccaa attgcctaaa tcagttgttg
     8821 ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt caaaaagcct
     8881 ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag cagaaaaaag
     8941 ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg cttaaaacgt
     9001 catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga gcgacagtat
     9061 tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca actgagtgta
     9121 tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga tgcgaccacg
     9181 ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca gaaagcataa
     9241 ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc tgataatcag
     9301 actgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc
     9361 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
     9421 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
     9481 catggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     9541 cgtcggtctg attattagtc tggaaccacg gtcccactcg tatcgtcggt ctgattatta
     9601 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacgatcc
     9661 cactcgtgtt gtcggtctga ttatcggtct gggaccacgg tcccacttgt attgtcgatc
     9721 agactatcag cgtgagacta cgattccatc aatgcctgtc aagggcaagt attgacatgt
     9781 cgtcgtaacc tgt
//
