LOCUS       sequence-56930-e        2676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Carlson LA, Hurley JH
  TITLE     In vitro reconstitution of the ordered assembly of the endosomal 
            sorting complex required for transport at membrane-bound HIV-1 Gag 
            clusters.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 
            10.1073/pnas.1211759109. Epub 2012 Oct 1.
  PUBMED    23027949
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211759109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-10-16- 16"; volume: "109"; issue: "42"; pages: 
            "16928-33"
COMMENT     Sequence Label: pGagMBPHis
FEATURES             Location/Qualifiers
     source          1..2676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1501..1521
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1537..2634
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2491..2500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2608..2631
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2656..2673
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggtgcac gtgcaagtgt gctgagcggt ggtgaactgg ataaatggga aaaaattcgt
       61 ctgcgcccgg gcggtaaaaa acagtacaaa ctgaaacata tcgtgtgggc gagccgtgaa
      121 ctggaacgct ttgccgttaa cccgggcctg ctggaaacct ctgaaggctg ccgtcagatt
      181 ctgggtcagc tgcagccgag cctgcagacg ggttctgaag aactgcgcag tctgtataac
      241 accattgcgg tgctgtactg tgttcaccag cgtatcgatg tgaaagatac gaaagaagcg
      301 ctggataaaa tcgaagaaga acagaacaaa agcaagaaaa aagcacagca ggcagcagca
      361 gataccggta acaattctca ggtgtctcag aactacccga ttgttcagaa tctgcagggt
      421 cagatggttc atcaggcaat cagtccgcgc accctgaacg cgtgggtgaa agtggttgaa
      481 gaaaaagcct ttagcccgga agttattccg atgttcagcg cactgtctga aggtgcaacg
      541 ccgcaggatc tgaacacgat gctgaatacc gtgggtggtc accaggcagc aatgcagatg
      601 ctgaaagaaa ccattaatga agaagcagcg gaatgggatc gtctgcatcc ggttcacgca
      661 ggtccgatcg caccgggtca gatgcgtgaa ccgcgtggta gcgatattgc gggtaccacg
      721 tctacgctgc aggaacagat cggctggatg acccataacc cgccgattcc ggtgggcgaa
      781 atctataaac gttggattat cctgggtctg aataaaattg ttcgcatgta cagtccgacg
      841 agcattctgg atattcgtca gggtccgaaa gaaccgtttc gtgattatgt ggatcgcttc
      901 tacaaaaccc tgcgcgcaga acaggcgtct caggaagtga aaaactggat gacggaaacc
      961 ctgctggttc agaacgccaa tccggattgc aaaaccatcc tgaaagcact gggcccgggt
     1021 gcgacgctgg aagaaatgat gaccgcatgt cagggtgtgg gtggtccggg tcataaagca
     1081 cgtgtgctgg cggaagccat gagccaggtt acgaatccgg caaccattat gatccagaaa
     1141 ggcaactttc gtaatcagcg caaaaccgtt aaatgcttca actgtggcaa agaaggtcac
     1201 attgccaaaa attgccgtgc accgcgcaaa aaaggctgct ggaaatgtgg caaagaaggt
     1261 catcagatga aagattgtac ggaacgccag gcaaactttc tgggtaaaat ctggccgagt
     1321 cacaaaggcc gtccgggtaa tttcctgcag agccgtccgg aaccgaccgc accgccggaa
     1381 gaatcttttc gcttcggcga agaaaccacg accccgagtc agcgtcagga accgattgat
     1441 aaagaactgt atccgctggc gtctctgcgt agtctgttcg gtagcgatcc gagctctcag
     1501 gaaaacctgt attttcaggg cggatccgga attcaaatga tcgaagaagg taaactggta
     1561 atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag
     1621 aaagataccg gaattaaagt caccgttgag catccggata aactggaaga gaaattccca
     1681 caggttgcgg caactggcga tggccctgac attatcttct gggcacacga ccgctttggt
     1741 ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag
     1801 ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc
     1861 gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc
     1921 tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg
     1981 ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg
     2041 ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg
     2101 aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc
     2161 gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc
     2221 ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg
     2281 accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc
     2341 gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa
     2401 ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag
     2461 gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa
     2521 atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc
     2581 aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg
     2641 agctcgtcac tcgagcacca ccaccaccac cactga
//
