LOCUS       sequence-56943-e       10759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH051
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1368..1396)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             2231..3421
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2583..2602)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3642..3662
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(join(3750..3756,3757..3774,3775..3780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3795..3816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4036..4695)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4610..4629
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4696..4798)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5656..6270
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5747..6278
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6346..6565
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6656..7411
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7414..7664
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7990..9165
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9165..10136
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10209..10682
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     1381 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1441 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1501 ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagc
     1561 caacacttgt cactactgct gtcgagatat gacggtgttc acaaagttcc ttaaatttta
     1621 cttttggtta catatttttt ctttttgaaa ccaaatcttt atctttgtag cactttcacg
     1681 gtagcgaaac gttagtttga atggaaagat gcctgcagac acataaagac accaaactct
     1741 catcaatagt tccgtaaatt tttattgaca gaacttattg acggcagtgg caggtgtcat
     1801 aaaaaaaacc atgagggtaa taaataatgg caacagttaa ccagctggta cgcaaaccac
     1861 gtgctcgcaa agttgcgaaa agcaacgtgc ctgcgctgga agcatgcccg caaaaacgtg
     1921 gcgtatgtac tcgtgtatat actaccactc ctaaaaaacc gaactccgcg ctgcgtaaag
     1981 tatgccgtgt tcgtctgact aacggtttcg aagtgacttc ctacatcggt ggtgaaggtc
     2041 acaacctgca ggagcactcc gtgatcctga tccgtggcgg tcgtgttaaa gacctcccgg
     2101 gtgttcgtta ccacaccgta cgtggtgcgc ttgactgctc cggcgttaaa gaccgtaagc
     2161 aggctcgttc caagtatggc gtgaagcgtc ctaaggctta atggttctcc aacgcagtca
     2221 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     2281 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
     2341 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
     2401 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
     2461 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
     2521 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     2581 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     2641 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     2701 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     2761 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     2821 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     2881 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     2941 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     3001 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     3061 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     3121 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     3181 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     3241 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     3301 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     3361 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     3421 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagattaaa ttttcagaac
     3481 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     3541 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     3601 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     3661 acagctgctg ggattacaca tggcatggat gaactataca aatagggtac cgcggccgcg
     3721 ctgaggggct ggccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgaacccctt
     3901 gcggccgccc gggccgtcga ccaattctca tgtttgacag cttatcatcg aatttctgcc
     3961 attcatccgc ttattatcac ttattcaggc gtagcaacca ggcgtttaag ggcaccaata
     4021 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4081 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     4141 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4201 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4261 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4321 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4381 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4441 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     4501 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4561 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4621 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4681 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     4741 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     4801 gtctcatttt cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt
     4861 tatttattct gcgaagtgat cttccgtcac aggtatttat tcgcgataag ctcatggagc
     4921 ggcgtaaccg tcgcacagga aggacagaga aagcgcggat ctgggaagtg acggacagaa
     4981 cggtcaggac ctggattggg gaggcggttg ccgccgctgc tgctgacggt gtgacgttct
     5041 ctgttccggt cacaccacat acgttccgcc attcctatgc gatgcacatg ctgtatgccg
     5101 gtataccgct gaaagttctg caaagcctga tgggacataa gtccatcagt tcaacggaag
     5161 tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca ccgggtgcag tttgcgatgc
     5221 cggagtctga tgcggttgcg atgctgaaac aattatcctg agaataaatg ccttggcctt
     5281 tatatggaaa tgtggaactg agtggatatg ctgtttttgt ctgttaaaca gagaagctgg
     5341 ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat ctcccattat cgtagagatc
     5401 cgcattatta atctcaggag cctgtgtagc gtttatagga agtagtgttc tgtcatgatg
     5461 cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag gaacaataga aatcttcgtg
     5521 cggtgttacg ttgaagtgga gcggattatg tcagcaatgg acagaacaac ctaatgaaca
     5581 cagaaccatg atgtggtctg tccttttaca gccagtagtg ctcgccgcag tcgagcgaca
     5641 gggcgaagcc ctcggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca
     5701 aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc gccggcgttg
     5761 tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg
     5821 aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc
     5881 gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc
     5941 acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc
     6001 gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg
     6061 aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa
     6121 gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat
     6181 atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa
     6241 ggggggtgcc cccccttctc gaaccctccc ggtcgagtga gcgaggaagc accagggaac
     6301 agcacttata tattctgctt acacacgatg cctgaaaaaa cttcccttgg ggttatccac
     6361 ttatccacgg ggatattttt ataattattt tttttatagt ttttagatct tcttttttag
     6421 agcgccttgt aggcctttat ccatgctggt tctagagaag gtgttgtgac aaattgccct
     6481 ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg cccttaaccc
     6541 tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa gttatccctg cttattgact
     6601 cttttttatt tagtgtgaca atctaaaaac ttgtcacact tcacatggat ctgtcatggc
     6661 ggaaacagcg gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg tccagtcaaa
     6721 cgacctcact gaggcggcat atagtctctc ccgggatcaa aaacgtatgc tgtatctgtt
     6781 cgttgaccag atcagaaaat ctgatggcac cctacaggaa catgacggta tctgcgagat
     6841 ccatgttgct aaatatgctg aaatattcgg attgacctct gcggaagcca gtaaggatat
     6901 acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc ctgaagagga
     6961 tgccggcgat gaaaaaggct atgaatcttt tccttggttt atcaaacgtg cgcacagtcc
     7021 atccagaggg ctttacagtg tacatatcaa cccatatctc attcccttct ttatcgggtt
     7081 acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca atccgtatgc
     7141 catgcgttta tacgaatccc tgtgtcagta tcgtaagccg gatggctcag gcatcgtctc
     7201 tctgaaaatc gactggatca tagagcgtta ccagctgcct caaagttacc agcgtatgcc
     7261 tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag atcaacagca gaactccaat
     7321 gcgcctctca tacattgaga aaaagaaagg ccgccagacg actcatatcg tattttcctt
     7381 ccgcgatatc acttccatga cgacaggata gtctgagggt tatctgtcac agatttgagg
     7441 gtggttcgtc acatttgttc tgacctactg agggtaattt gtcacagttt tgctgtttcc
     7501 ttcagcctgc atggattttc tcatactttt tgaactgtaa tttttaagga agccaaattt
     7561 gagggcagtt tgtcacagtt gatttccttc tctttccctt cgtcatgtga cctgatatcg
     7621 ggggttagtt cgtcatcatt gatgagggtt gattatcaca gtttattact ctgaattggc
     7681 tatccgcgtg tgtacctcta cctggagttt ttcccacggt ggatatttct tcttgcgctg
     7741 agcgtaagag ctatctgaca gaacagttct tctttgcttc ctcgccagtt cgctcgctat
     7801 gctcggttac acggctgcgg cgagcgctag tgataataag tgactgaggt atgtgctctt
     7861 cttatctcct tttgtagtgt tgctcttatt ttaaacaact ttgcggtttt ttgatgactt
     7921 tgcgattttg ttgttgcttt gcagtaaatt gcaagattta ataaaaaaac gcaaagcaat
     7981 gattaaagga tgttcagaat gaaactcatg gaaacactta accagtgcat aaacgctggt
     8041 catgaaatga cgaaggctat cgccattgca cagtttaatg atgacagccc ggaagcgagg
     8101 aaaataaccc ggcgctggag aataggtgaa gcagcggatt tagttggggt ttcttctcag
     8161 gctatcagag atgccgagaa agcagggcga ctaccgcacc cggatatgga aattcgagga
     8221 cgggttgagc aacgtgttgg ttatacaatt gaacaaatta atcatatgcg tgatgtgttt
     8281 ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg tgatcggggt tgctgcccat
     8341 aaaggtggcg tttacaaaac ctcagtttct gttcatcttg ctcaggatct ggctctgaag
     8401 gggctacgtg ttttgctcgt ggaaggtaac gacccccagg gaacagcctc aatgtatcac
     8461 ggatgggtac cagatcttca tattcatgca gaagacactc tcctgccttt ctatcttggg
     8521 gaaaaggacg atgtcactta tgcaataaag cccacttgct ggccggggct tgacattatt
     8581 ccttcctgtc tggctctgca ccgtattgaa actgagttaa tgggcaaatt tgatgaaggt
     8641 aaactgccca ccgatccaca cctgatgctc cgactggcca ttgaaactgt tgctcatgac
     8701 tatgatgtca tagttattga cagcgcgcct aacctgggta tcggcacgat taatgtcgta
     8761 tgtgctgctg atgtgctgat tgttcccacg cctgctgagt tgtttgacta cacctccgca
     8821 ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg ttgatcttaa agggttcgag
     8881 cctgatgtac gtattttgct taccaaatac agcaatagta atggctctca gtccccgtgg
     8941 atggaggagc aaattcggga tgcctgggga agcatggttc taaaaaatgt tgtacgtgaa
     9001 acggatgaag ttggtaaagg tcagatccgg atgagaactg tttttgaaca ggccattgat
     9061 caacgctctt caactggtgc ctggagaaat gctctttcta tttgggaacc tgtctgcaat
     9121 gaaattttcg atcgtctgat taaaccacgc tgggagatta gataatgaag cgtgcgcctg
     9181 ttattccaaa acatacgctc aatactcaac cggttgaaga tacttcgtta tcgacaccag
     9241 ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt aatggctcgc ggtaatgcca
     9301 ttactttgcc tgtatgtggt cgggatgtga agtttactct tgaagtgctc cggggtgata
     9361 gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg tgaccaggag ctgcttactg
     9421 aggacgcact ggatgatctc atcccttctt ttctactgac tggtcaacag acaccggcgt
     9481 tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg gagtcgccgt cgtaaagctg
     9541 ctgcacttac cgaaagtgat tatcgtgttc tggttggcga gctggatgat gagcagatgg
     9601 ctgcattatc cagattgggt aacgattatc gcccaacaag tgcttatgaa cgtggtcagc
     9661 gttatgcaag ccgattgcag aatgaatttg ctggaaatat ttctgcgctg gctgatgcgg
     9721 aaaatatttc acgtaagatt attacccgct gtatcaacac cgccaaattg cctaaatcag
     9781 ttgttgctct tttttctcac cccggtgaac tatctgcccg gtcaggtgat gcacttcaaa
     9841 aagcctttac agataaagag gaattactta agcagcaggc atctaacctt catgagcaga
     9901 aaaaagctgg ggtgatattt gaagctgaag aagttatcac tcttttaact tctgtgctta
     9961 aaacgtcatc tgcatcaaga actagtttaa gctcacgaca tcagtttgct cctggagcga
    10021 cagtattgta taagggcgat aaaatggtgc ttaacctgga caggtctcgt gttccaactg
    10081 agtgtataga gaaaattgag gccattctta aggaacttga aaagccagca ccctgatgcg
    10141 accacgtttt agtctacgtt tatctgtctt tacttaatgt cctttgttac aggccagaaa
    10201 gcataactgg cctgaatatt ctctctgggc ccactgttcc acttgtatcg tcggtctgat
    10261 aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac
    10321 ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt
    10381 cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
    10441 tgggaccatg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
    10501 tcgtatcgtc ggtctgatta ttagtctgga accacggtcc cactcgtatc gtcggtctga
    10561 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
    10621 cgatcccact cgtgttgtcg gtctgattat cggtctggga ccacggtccc acttgtattg
    10681 tcgatcagac tatcagcgtg agactacgat tccatcaatg cctgtcaagg gcaagtattg
    10741 acatgtcgtc gtaacctgt
//
