LOCUS       sequence-56944-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a CMV promoter when no GFP tracer is desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle-CMV
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    226..347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(263..282)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     317..336
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tatataagca gagctggttt agtgaaccgt cagatccgct agagatctgg taccgtcgac
       61 gcggccgctc gagcctaagc ttctagataa gatatccgat ccaccggatc tagataactg
      121 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      181 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      241 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      301 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cggatctggg
      361 cgtggttaag ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt
      421 ttgcagcagc cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat
      481 atttgacaac gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca
      541 ttgatggtcg ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg
      601 gaacgccgtt ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg
      661 ggattgtgac tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat
      721 ccgcccgcga tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac
      781 ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt
      841 cctcccctcc caatgcggtt taaaa
//
