LOCUS       sequence-56946-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet27
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta actttaataa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggtgccgc gcggcagcct aggacagtac aaacttatcc tgaacggtaa
      121 aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct gctaccgcgg aaaaagtttt
      181 caaacagtac gctaacgaca acggtgttga cggtgaatgg acctacgacg acgctaccaa
      241 aacctacacc gtaacggaag gatccaaatg tgtgtctggc gatacaccgg tacttctgga
      301 tgccggcgag aggaggatag gcgacctgtt catggaggcc attcaggacc aaaagaacgc
      361 ggtcgaaata ggccagaacg aagagatagt ccggctccat gatccgctgc gcatatattc
      421 catggtcggt tctgaaatag tcgaaagcgt ctctcacgcc atatatcacg gaaagagcaa
      481 tgccattgta accgttagga cggagaatgg aagagaggtc agggtgacac ctgtccacaa
      541 actctttgtt aaaattggaa actctgtaat cgagaggcca gcctcagagg tgaatgaggg
      601 cgatgaaata gcatgcgcaa gcgtaagtga gaacggtgat tcccaaaccg tcaccacaac
      661 gctggtattg acattcgata gagtggtatc aaaggaaatg catagcggcg tattcgatgt
      721 ctacgatctg atggttccgg attatggata caacttcata ggcggaaatg gcctcatagt
      781 ccttcacaac accgtgatag gtacccagta caaacttatc ctgaacggta aaaccctgaa
      841 aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg g
//
