LOCUS       sequence-56947-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Ellila S, Jurvansuu JM, Iwai H
  TITLE     Evaluation and comparison of protein splicing by exogenous inteins 
            with foreign exteins in Escherichia coli.
  JOURNAL   FEBS Lett. 2011 Oct 10.
  PUBMED    22001202
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2011 Oct 10."
COMMENT     Sequence Label: pSKDuet27
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagcc
       61 gccaaaacag ccaagcttat tccgttacgg tgtaggtttt ggtagcgtcg tcgtaggtcc
      121 attcaccgtc aacaccgttg tcgttagcgt actgtttgaa aactttttcc gcggtagcag
      181 cgtcaacagc ttcggtggtg gtttcacctt tcagggtttt accgttcagg ataagtttgt
      241 actgggtacc tatcacggtg ttgtgaagga ctatgaggcc atttccgcct atgaagttgt
      301 atccataatc cggaaccatc agatcgtaga catcgaatac gccgctatgc atttcctttg
      361 ataccactct atcgaatgtc aataccagcg ttgtggtgac ggtttgggaa tcaccgttct
      421 cacttacgct tgcgcatgct atttcatcgc cctcattcac ctctgaggct ggcctctcga
      481 ttacagagtt tccaatttta acaaagagtt tgtggacagg tgtcaccctg acctctcttc
      541 cattctccgt cctaacggtt acaatggcat tgctctttcc gtgatatatg gcgtgagaga
      601 cgctttcgac tatttcagaa ccgaccatgg aatatatgcg cagcggatca tggagccgga
      661 ctatctcttc gttctggcct atttcgaccg cgttcttttg gtcctgaatg gcctccatga
      721 acaggtcgcc tatcctcctc tcgccggcat ccagaagtac cggtgtatcg ccagacacac
      781 atttggatcc ttccgttacg gtgtaggttt tggtagcgt
//
