LOCUS       sequence-56950-e       10764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH053
FEATURES             Location/Qualifiers
     source          1..10764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2231..3421
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2583..2602)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(3755..3761,3762..3779,3780..3785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3800..3821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4041..4700)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4615..4634
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4701..4803)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5661..6275
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5752..6283
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6351..6570
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6661..7416
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7419..7669
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             7995..9170
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9170..10141
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10214..10687
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaatg gtctcaaagg gtgaagaaga taacatggca attattaaag
     1381 agtttatgcg tttcaaggtg catatggagg gatctgtcaa tgggcatgag tttgaaattg
     1441 aaggtgaagg agaaggccga ccatatgagg gaacacaaac cgcaaaacta aaggtaagtt
     1501 taaacatata tatactaact aaccctgatt atttaaattt tcaggtaact aaaggcggac
     1561 cattaccatt cgcctgggct gtcgagatat gacggtgttc acaaagttcc ttaaatttta
     1621 cttttggtta catatttttt ctttttgaaa ccaaatcttt atctttgtag cactttcacg
     1681 gtagcgaaac gttagtttga atggaaagat gcctgcagac acataaagac accaaactct
     1741 catcaatagt tccgtaaatt tttattgaca gaacttattg acggcagtgg caggtgtcat
     1801 aaaaaaaacc atgagggtaa taaataatgg caacagttaa ccagctggta cgcaaaccac
     1861 gtgctcgcaa agttgcgaaa agcaacgtgc ctgcgctgga agcatgcccg caaaaacgtg
     1921 gcgtatgtac tcgtgtatat actaccactc ctaaaaaacc gaactccgcg ctgcgtaaag
     1981 tatgccgtgt tcgtctgact aacggtttcg aagtgacttc ctacatcggt ggtgaaggtc
     2041 acaacctgca ggagcactcc gtgatcctga tccgtggcgg tcgtgttaaa gacctcccgg
     2101 gtgttcgtta ccacaccgta cgtggtgcgc ttgactgctc cggcgttaaa gaccgtaagc
     2161 aggctcgttc caagtatggc gtgaagcgtc ctaaggctta atggttctcc aacgcagtca
     2221 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     2281 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
     2341 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
     2401 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
     2461 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
     2521 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     2581 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     2641 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     2701 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     2761 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     2821 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     2881 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     2941 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     3001 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     3061 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     3121 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     3181 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     3241 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     3301 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     3361 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     3421 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagacatta cgatgctgag
     3481 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca ggtgaagaca
     3541 acttacaaag ccaaaaaacc agttcagctg ccaggagcgt acaatgttaa tattaaactg
     3601 gatatcacct cccacaacga ggattacact atcgttgagc aatatgaaag agctgaaggg
     3661 cggcactcga caggtggcat ggatgaattg tataagacta gtggatccta agtaccgcgg
     3721 ccgcgctgag gggctggccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgaac
     3901 cccttgcggc cgcccgggcc gtcgaccaat tctcatgttt gacagcttat catcgaattt
     3961 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac
     4021 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     4081 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     4141 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     4201 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     4261 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     4321 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     4381 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     4441 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     4501 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     4561 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     4621 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     4681 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     4741 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     4801 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     4861 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcgcg ataagctcat
     4921 ggagcggcgt aaccgtcgca caggaaggac agagaaagcg cggatctggg aagtgacgga
     4981 cagaacggtc aggacctgga ttggggaggc ggttgccgcc gctgctgctg acggtgtgac
     5041 gttctctgtt ccggtcacac cacatacgtt ccgccattcc tatgcgatgc acatgctgta
     5101 tgccggtata ccgctgaaag ttctgcaaag cctgatggga cataagtcca tcagttcaac
     5161 ggaagtctac acgaaggttt ttgcgctgga tgtggctgcc cggcaccggg tgcagtttgc
     5221 gatgccggag tctgatgcgg ttgcgatgct gaaacaatta tcctgagaat aaatgccttg
     5281 gcctttatat ggaaatgtgg aactgagtgg atatgctgtt tttgtctgtt aaacagagaa
     5341 gctggctgtt atccactgag aagcgaacga aacagtcggg aaaatctccc attatcgtag
     5401 agatccgcat tattaatctc aggagcctgt gtagcgttta taggaagtag tgttctgtca
     5461 tgatgcctgc aagcggtaac gaaaacgatt tgaatatgcc ttcaggaaca atagaaatct
     5521 tcgtgcggtg ttacgttgaa gtggagcgga ttatgtcagc aatggacaga acaacctaat
     5581 gaacacagaa ccatgatgtg gtctgtcctt ttacagccag tagtgctcgc cgcagtcgag
     5641 cgacagggcg aagccctcgg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     5701 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cggccgccgg
     5761 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     5821 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     5881 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     5941 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     6001 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     6061 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     6121 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     6181 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     6241 ccgaaggggg gtgccccccc ttctcgaacc ctcccggtcg agtgagcgag gaagcaccag
     6301 ggaacagcac ttatatattc tgcttacaca cgatgcctga aaaaacttcc cttggggtta
     6361 tccacttatc cacggggata tttttataat tatttttttt atagttttta gatcttcttt
     6421 tttagagcgc cttgtaggcc tttatccatg ctggttctag agaaggtgtt gtgacaaatt
     6481 gccctttcag tgtgacaaat caccctcaaa tgacagtcct gtctgtgaca aattgccctt
     6541 aaccctgtga caaattgccc tcagaagaag ctgttttttc acaaagttat ccctgcttat
     6601 tgactctttt ttatttagtg tgacaatcta aaaacttgtc acacttcaca tggatctgtc
     6661 atggcggaaa cagcggttat caatcacaag aaacgtaaaa atagcccgcg aatcgtccag
     6721 tcaaacgacc tcactgaggc ggcatatagt ctctcccggg atcaaaaacg tatgctgtat
     6781 ctgttcgttg accagatcag aaaatctgat ggcaccctac aggaacatga cggtatctgc
     6841 gagatccatg ttgctaaata tgctgaaata ttcggattga cctctgcgga agccagtaag
     6901 gatatacggc aggcattgaa gagtttcgcg gggaaggaag tggtttttta tcgccctgaa
     6961 gaggatgccg gcgatgaaaa aggctatgaa tcttttcctt ggtttatcaa acgtgcgcac
     7021 agtccatcca gagggcttta cagtgtacat atcaacccat atctcattcc cttctttatc
     7081 gggttacaga accggtttac gcagtttcgg cttagtgaaa caaaagaaat caccaatccg
     7141 tatgccatgc gtttatacga atccctgtgt cagtatcgta agccggatgg ctcaggcatc
     7201 gtctctctga aaatcgactg gatcatagag cgttaccagc tgcctcaaag ttaccagcgt
     7261 atgcctgact tccgccgccg cttcctgcag gtctgtgtta atgagatcaa cagcagaact
     7321 ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc agacgactca tatcgtattt
     7381 tccttccgcg atatcacttc catgacgaca ggatagtctg agggttatct gtcacagatt
     7441 tgagggtggt tcgtcacatt tgttctgacc tactgagggt aatttgtcac agttttgctg
     7501 tttccttcag cctgcatgga ttttctcata ctttttgaac tgtaattttt aaggaagcca
     7561 aatttgaggg cagtttgtca cagttgattt ccttctcttt cccttcgtca tgtgacctga
     7621 tatcgggggt tagttcgtca tcattgatga gggttgatta tcacagttta ttactctgaa
     7681 ttggctatcc gcgtgtgtac ctctacctgg agtttttccc acggtggata tttcttcttg
     7741 cgctgagcgt aagagctatc tgacagaaca gttcttcttt gcttcctcgc cagttcgctc
     7801 gctatgctcg gttacacggc tgcggcgagc gctagtgata ataagtgact gaggtatgtg
     7861 ctcttcttat ctccttttgt agtgttgctc ttattttaaa caactttgcg gttttttgat
     7921 gactttgcga ttttgttgtt gctttgcagt aaattgcaag atttaataaa aaaacgcaaa
     7981 gcaatgatta aaggatgttc agaatgaaac tcatggaaac acttaaccag tgcataaacg
     8041 ctggtcatga aatgacgaag gctatcgcca ttgcacagtt taatgatgac agcccggaag
     8101 cgaggaaaat aacccggcgc tggagaatag gtgaagcagc ggatttagtt ggggtttctt
     8161 ctcaggctat cagagatgcc gagaaagcag ggcgactacc gcacccggat atggaaattc
     8221 gaggacgggt tgagcaacgt gttggttata caattgaaca aattaatcat atgcgtgatg
     8281 tgtttggtac gcgattgcga cgtgctgaag acgtatttcc accggtgatc ggggttgctg
     8341 cccataaagg tggcgtttac aaaacctcag tttctgttca tcttgctcag gatctggctc
     8401 tgaaggggct acgtgttttg ctcgtggaag gtaacgaccc ccagggaaca gcctcaatgt
     8461 atcacggatg ggtaccagat cttcatattc atgcagaaga cactctcctg cctttctatc
     8521 ttggggaaaa ggacgatgtc acttatgcaa taaagcccac ttgctggccg gggcttgaca
     8581 ttattccttc ctgtctggct ctgcaccgta ttgaaactga gttaatgggc aaatttgatg
     8641 aaggtaaact gcccaccgat ccacacctga tgctccgact ggccattgaa actgttgctc
     8701 atgactatga tgtcatagtt attgacagcg cgcctaacct gggtatcggc acgattaatg
     8761 tcgtatgtgc tgctgatgtg ctgattgttc ccacgcctgc tgagttgttt gactacacct
     8821 ccgcactgca gtttttcgat atgcttcgtg atctgctcaa gaacgttgat cttaaagggt
     8881 tcgagcctga tgtacgtatt ttgcttacca aatacagcaa tagtaatggc tctcagtccc
     8941 cgtggatgga ggagcaaatt cgggatgcct ggggaagcat ggttctaaaa aatgttgtac
     9001 gtgaaacgga tgaagttggt aaaggtcaga tccggatgag aactgttttt gaacaggcca
     9061 ttgatcaacg ctcttcaact ggtgcctgga gaaatgctct ttctatttgg gaacctgtct
     9121 gcaatgaaat tttcgatcgt ctgattaaac cacgctggga gattagataa tgaagcgtgc
     9181 gcctgttatt ccaaaacata cgctcaatac tcaaccggtt gaagatactt cgttatcgac
     9241 accagctgcc ccgatggtgg attcgttaat tgcgcgcgta ggagtaatgg ctcgcggtaa
     9301 tgccattact ttgcctgtat gtggtcggga tgtgaagttt actcttgaag tgctccgggg
     9361 tgatagtgtt gagaagacct ctcgggtatg gtcaggtaat gaacgtgacc aggagctgct
     9421 tactgaggac gcactggatg atctcatccc ttcttttcta ctgactggtc aacagacacc
     9481 ggcgttcggt cgaagagtat ctggtgtcat agaaattgcc gatgggagtc gccgtcgtaa
     9541 agctgctgca cttaccgaaa gtgattatcg tgttctggtt ggcgagctgg atgatgagca
     9601 gatggctgca ttatccagat tgggtaacga ttatcgccca acaagtgctt atgaacgtgg
     9661 tcagcgttat gcaagccgat tgcagaatga atttgctgga aatatttctg cgctggctga
     9721 tgcggaaaat atttcacgta agattattac ccgctgtatc aacaccgcca aattgcctaa
     9781 atcagttgtt gctctttttt ctcaccccgg tgaactatct gcccggtcag gtgatgcact
     9841 tcaaaaagcc tttacagata aagaggaatt acttaagcag caggcatcta accttcatga
     9901 gcagaaaaaa gctggggtga tatttgaagc tgaagaagtt atcactcttt taacttctgt
     9961 gcttaaaacg tcatctgcat caagaactag tttaagctca cgacatcagt ttgctcctgg
    10021 agcgacagta ttgtataagg gcgataaaat ggtgcttaac ctggacaggt ctcgtgttcc
    10081 aactgagtgt atagagaaaa ttgaggccat tcttaaggaa cttgaaaagc cagcaccctg
    10141 atgcgaccac gttttagtct acgtttatct gtctttactt aatgtccttt gttacaggcc
    10201 agaaagcata actggcctga atattctctc tgggcccact gttccacttg tatcgtcggt
    10261 ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
    10321 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
    10381 atcgtcggtc tgataatcag actgggacca cggtcccact cgtatcgtcg gtctgattat
    10441 tagtctggga ccatggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt
    10501 cccactcgta tcgtcggtct gattattagt ctggaaccac ggtcccactc gtatcgtcgg
    10561 tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg
    10621 gaccacgatc ccactcgtgt tgtcggtctg attatcggtc tgggaccacg gtcccacttg
    10681 tattgtcgat cagactatca gcgtgagact acgattccat caatgcctgt caagggcaag
    10741 tattgacatg tcgtcgtaac ctgt
//
