LOCUS       sequence-56961-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Kodama Y, Hu CD
  TITLE     An improved bimolecular fluorescence complementation assay with a 
            high signal-to-noise ratio.
  JOURNAL   Biotechniques. 2010 Nov . 49(5):793-805.
  PUBMED    21091444
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Nov . 49(5):793-805."
COMMENT     Sequence Label: pBiFC-VN155(I152L)
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    93..227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(130..149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     184..203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(242..700,701..703,704..706))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     381..400
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     640..661
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcatgcctgc agatcgactc tagaggatcc gaaaaaacct cccacacctc cccctgaacc
       61 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc gcggccgctc
      241 aggcggtgag atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      301 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      361 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      421 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
      481 ggtagcgggc gaagcactgc aggccgtagc ccagggtggt cacgagggtg ggccagggca
      541 cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc
      601 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      661 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatactc ccgccacctc
      721 cgctcccgcc acctccggta cctcgagaga tctcggtcga ccgaattcgg gcctccatgg
      781 ccataagcag gtcctcctct gagatcagct tctgcattga tgccatggtg ggcccgcggg
      841 tacaattccg cagcttttag agcagaagta acacttccgt acaggcctag aagtaaaggc
      901 aacatccact gaggagcagt tctttgattt gcaccaccac cggatccggg ac
//
