LOCUS       sequence-56966-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kodama Y, Hu CD
  TITLE     An improved bimolecular fluorescence complementation assay with a 
            high signal-to-noise ratio.
  JOURNAL   Biotechniques. 2010 Nov . 49(5):793-805.
  PUBMED    21091444
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Nov . 49(5):793-805."
COMMENT     Sequence Label: pBiFC-VN155(I152L)
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          105..201
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(427..448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(688..707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
       61 gaggatccgg tactagagga actgaaaaac cagaaagtta actggtaagt ttagtctttt
      121 tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt
      181 ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg
      241 aattgtaccc gcgggcccac catggcatca atgcagaagc tgatctcaga ggaggacctg
      301 cttatggcca tggaggcccg aattcggtcg accgagatct ctcgaggtac cggaggtggc
      361 gggagcggag gtggcgggag tatggtgagc aagggcgagg agctgttcac cggggtggtg
      421 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      481 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
      541 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
      601 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      661 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      721 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
//
