LOCUS       sequence-56968-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength intracellular glutamate sensor constructed from 
            E. coli Gltl and cpGFP. For bacterial cytoplasmic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, 
            Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter 
            ER, Demb JB, Gan WB, Hires SA, Looger LL
  TITLE     An optimized fluorescent probe for visualizing glutamate 
            neurotransmission.
  JOURNAL   Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 
            2013 Jan 13.
  PUBMED    23314171
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2333"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "162-70"
COMMENT     Sequence Label: pRSET.GltI253-cpGFP.L1LV/L2NP
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt cgaaagtttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
      241 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cctctgcttg tcggccatga
      301 tatagacgtt gtggctcacc agaattggat ttttgaacca cttatcaaac catttttccg
      361 cttcaccgga ggtctgcacc tgagcgatgg tgtcatccat cagctttttg aactgcggat
      421 catctttacg caacatacaa ccgtaggcct cctgagactg cggcttgccg acgatttccc
      481 agttgtctgg tttcttcgct ttcgcacgtt caccggccag cagcacgtca tccatcataa
      541 aggcaacggc acgaccgctt tccagggtgc ggaaagagtc accgtgatct ttggcgctga
      601 tgatgcgcat attcattttt tgctcttcat tcagtttgtt gagcaaaact tcagaggtag
      661 tgccggaagt gacgactacg gctttgtctt tcaggttggc aaaatctttg atatcgccac
      721 cctttttggt caacaggcgc gtaccgacca cgaaaatagt gtcagagaaa gccgcctgtt
      781 tttggcgttc gacgttgttg gtggtagaac cacattcaaa atcgaaagtg ccgttttgca
      841 gcagtggaat acggttttgt gaggtaatcg gaatcagttt tacctgcaag tccggtttgt
      901 tgagtttctt tttcactgct tcaacaatgg cgttggagta atcctgcgag taacccacca
      961 ctttttgctg attgtc
//
