LOCUS       sequence-56970-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength intracellular glutamate sensor constructed from 
            E. coli Gltl and cpGFP. For bacterial cytoplasmic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, 
            Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter 
            ER, Demb JB, Gan WB, Hires SA, Looger LL
  TITLE     An optimized fluorescent probe for visualizing glutamate 
            neurotransmission.
  JOURNAL   Nat Methods. 2013 Feb;10(2):162-70. doi: 10.1038/nmeth.2333. Epub 
            2013 Jan 13.
  PUBMED    23314171
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2333"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "162-70"
COMMENT     Sequence Label: pRSET.GltI253-cpGFP.L1LV/L2NP
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     235..254
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     494..515
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(621..642)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcaagct tcgaattctt atttcagtgc cttgtcattc
       61 ggttctttga acagtgcttt catttcgtct gacagttcga aattcatgtt cagggggttg
      121 ttaaagttgt actccagctt atgccccagg atgttgccgt cctccttgaa gtcgatgccc
      181 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      241 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga tgtagccttc gggcatggcg
      301 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
      361 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      421 aacttcaggg tcagcttgcc gtaggtggca tcgccctcac cctcgccgga cacgctgaac
      481 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      541 tcctcgccct tgctcaccat gctccctccg gtaccgccct tgtacagctc gtccatgccg
      601 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      661 gggtctttcg aaagtttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
      721 ccgtcgccga tgggggtgtt ctgctggtag tggtaggcga gctgcacgcc gccgtcctcg
      781 atgttgtggc ggatcttgaa gttcgccttg atgccgttcc tctgcttgtc ggccatgata
      841 tagacgttgt ggctcaccag aattggattt ttgaaccact tatcaaacca tttttccgct
      901 tcaccggagg tctgcacctg agcgatggtg tcatccatca gctttttgaa ctgcggatca
      961 tctttacgca acatacaacc gtaggcctcc tgagactgcg gcttgcc
//
