LOCUS       sequence-56975-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: IL2 F42A pAcGP67a
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     126..185
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             594..611
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
       61 ggcgcggatc tatgctacta gtaaatcagt cacaccaagg cttcaataag gaacacacaa
      121 gcaagatggt aagcgctatt gttttatatg tgcttttggc ggcggcggcg cattctgcct
      181 ttgcggcgga tccagcacct acttcaagtt ctacaaagaa aacacagcta caactggagc
      241 atttacttct ggatttacag atgattttga atggaattaa taattacaag aatcccaaac
      301 tcaccaggat gctcacagca aagttttaca tgcccaagaa ggccacagaa ctgaaacatc
      361 ttcagtgtct agaagaagaa ctcaaacctc tggaggaagt gctaaattta gctcaaagca
      421 aaaactttca cttaagaccc agggacttaa tcagcaatat caacgtaata gttctggaac
      481 taaagggatc tgaaacaaca ttcatgtgtg aatatgctga tgagacagca accattgtag
      541 aatttctgaa cagatggatt accttttgtc aaagcatcat ctcaacacta actcatcatc
      601 accatcacca ttgagaattc cggagcggcc gctgcagatc tgatcctttc ctgggacccg
      661 gcaagaacca aaaactcact ctcttcaagg aaatccgtaa tgttaaaccc gacacgatga
      721 agcttgtcgt tggatggaaa ggaaaagagt tctacaggga aacttggacc cgcttcatgg
      781 aagacagctt ccccattgtt aacgaccaag aagtgatgga tgttt
//
