LOCUS       sequence-56989-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyNT
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..136
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      233..279
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtctcgcagc gccgtcagtc accaaccctc caccggccgc gctcgcggcc accacccgat
       61 aagagaaagg gagtccacca tgaagcacca ccaccaccac cacccgtcgg ccggcgagaa
      121 cctgtacttc cagggcgcca tggacgataa ggtaccggat ccgaattcga tatcgctagc
      181 aagcttatcg atgtcgacgt agttaactag cgtacgatcg actgccaggc atcaaataaa
      241 acgaaaggct cagtcgaaag actgggcctt tcgttttatg ccatcatggc cgcggtgatc
      301 agctagaggg ggcgtcaggc gccgggggcg gtgtccggcg gcccccagag gaactgcgcc
      361 agttcctccg gatcggtgaa gccggagaga tccagcgggg tctcctcgaa cacctcgaag
      421 tcgtgcagga aggtgaaggc gagcagttcg cgggcgaagt cctcggtccg cttccactgc
      481 gccccgtcga gcagcgcggc caggatctcg cggtcgcccc ggaaggcgtt gagatgcagt
      541 tgcaccaggc tgtagcggga gtctcccgca tagacgtcgg tgaagtcgac gatcccggtg
      601 acctcggtcg cggccaggtc cacgaagatg ttggtcccgt gcaggtcgcc gtggacgaac
      661 cggggttcgc ggccggccag cagcgtgtcc acgtccggca gccagtcctc caggcggtcc
      721 agcagccggg gcgagaggta gccccacccg cggtggtcct cgacggtcgc cgcgcggcgt
      781 tcccgcagca gttccgggaa gacctcggaa tggggggtga gcacggtgtt cccggtcagc
      841 ggcaccctgt gcagccggcc gagcacccgg ccgagttcgc gggccagggc gagcagcgcg
      901 ttccggtcgg tcgtgccgtc catcgcggac cgccaggtgg tgccggtcat ccggctcatc
      961 accaggtagg gccacggcca ggctccggtg ccgggccgca gctcgccgcg gccgaggagg
     1021 cggggcaccg gcaccggggc gtccgccagg accgcgtacg cctccgactc cgacgcgagg
     1081 ctc
//
