LOCUS       sequence-56991-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: IL2 pMalLP
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(445..468)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(520..543)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gtcgacttaa tggtgatggt gatgatgagt tagtgttgag atgatgcttt gacaaaaggt
       61 aatccatctg ttcagaaatt ctacaatggt tgctgtctca tcagcatatt cacacatgaa
      121 tgttgtttca gatcccttta gttccagaac tattacgttg atattgctga ttaagtccct
      181 gggtcttaag tgaaagtttt tgctttgagc taaatttagc acttcctcca gaggtttgag
      241 ttcttcttct agacactgaa gatgtttcag ttctgtggcc ttcttgggca tgtaaaactt
      301 aaatgtgagc atcctggtga gtttgggatt cttgtaatta ttaattccat tcaaaatcat
      361 ctgtaaatcc agaagtaaat gctccagttg tagctgtgtt ttctttgtag aacttgaagt
      421 aggtgcgaat tctcccatgg ctccgggccc ctggaacaga acttccagcc cgaggttgtt
      481 gttattgtta ttgttgttgt tgttcgagct cgaattagtc tgcgcgtctt tcagggcttc
      541 atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
      601 agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt ccatagtggc
      661 ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcta cggcacccag
      721 cggtttgtct ttattaaccg cttccagacc ttcatcagtc agcagatagt tttcgaggaa
      781 ctcttttgcc agctctttgt tcggactggc ggcgttaata cctgcgctca gcacgccaac
      841 gaacg
//
