LOCUS       sequence-57001-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyC
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             132..149
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      208..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgtctcgcag cgccgtcagt caccaaccct ccaccggccg cgctcgcggc caccacccga
       61 taagagaaag ggagtccacc atgggctcgg ccggtaccgg atccgaattc gatatcgcta
      121 gccaagcttc gcaccaccac caccaccact gataacagct tatcgatgtc gacgtagtta
      181 actagcgtac gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      241 gcctttcgtt ttatgccatc atggccgcgg tgatcagcta gagggggcgt caggcgccgg
      301 gggcggtgtc cggcggcccc cagaggaact gcgccagttc ctccggatcg gtgaagccgg
      361 agagatccag cggggtctcc tcgaacacct cgaagtcgtg caggaaggtg aaggcgagca
      421 gttcgcgggc gaagtcctcg gtccgcttcc actgcgcccc gtcgagcagc gcggccagga
      481 tctcgcggtc gccccggaag gcgttgagat gcagttgcac caggctgtag cgggagtctc
      541 ccgcatagac gtcggtgaag tcgacgatcc cggtgacctc ggtcgcggcc aggtccacga
      601 agatgttggt cccgtgcagg tcgccgtgga cgaaccgggg ttcgcggccg gccagcagcg
      661 tgtccacgtc cggcagccag tcctccaggc ggtccagcag ccggggcgag aggtagcccc
      721 acccgcggtg gtcctcgacg gtcgccgcgc ggcgttcccg cagcagttcc gggaagacct
      781 cggaatgggg ggtgagcacg gtgttcccgg tcagcggcac cctgtgcagc cggccgagca
      841 cccggccgag ttcgcgggcc agggcgagca gcgcgttccg gtcggtcgtg ccgtccatcg
      901 cggaccgcca ggtggtgccg gtcatccggc tcatcaccag gtagggccac ggccaggctc
      961 cggtgccggg ccgcagctcg ccgcggccga ggaggcgggg caccggcacc ggggcgtccg
     1021 ccaggaccgc gtacgcctcc gactccgacg cgaggctc
//
