LOCUS       sequence-57003-e       10965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH066
FEATURES             Location/Qualifiers
     source          1..10965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1348..1392
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1408..1431
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1447..1470
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2425..3615
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2777..2796)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(3956..3962,3963..3980,3981..3986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4001..4022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4242..4901)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4816..4835
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4902..5004)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5862..6476
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5953..6484
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6552..6771
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6862..7617
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7620..7870
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8196..9371
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9371..10342
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10415..10888
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaaac catggttaaa gaaacagcag cagcgaaatt tgaaagacaa
     1381 catatggata gtggaggcgg tggaagtttg gaagttcttt tccaaggacc gggcggagga
     1441 ggctcttggt cgcatccaca gttcgaaaaa gccatggccg gcggtggtgg atcgggagga
     1501 ggaggttcgg gtggcggagg atcgggcgcc tcaaaaggag aagaaacgac aatgggtgtt
     1561 atcaagccag acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc
     1621 gtcattgagg gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa
     1681 gtgaaggagg gtgccccact tccattctct tatgatatcc ttactacagc cttcgcatat
     1741 ggaaatcgag cattcacaaa atacccagac ggctgtcgag atatgacggt gttcacaaag
     1801 ttccttaaat tttacttttg gttacatatt ttttcttttt gaaaccaaat ctttatcttt
     1861 gtagcacttt cacggtagcg aaacgttagt ttgaatggaa agatgcctgc agacacataa
     1921 agacaccaaa ctctcatcaa tagttccgta aatttttatt gacagaactt attgacggca
     1981 gtggcaggtg tcataaaaaa aaccatgagg gtaataaata atggcaacag ttaaccagct
     2041 ggtacgcaaa ccacgtgctc gcaaagttgc gaaaagcaac gtgcctgcgc tggaagcatg
     2101 cccgcaaaaa cgtggcgtat gtactcgtgt atatactacc actcctaaaa aaccgaactc
     2161 cgcgctgcgt aaagtatgcc gtgttcgtct gactaacggt ttcgaagtga cttcctacat
     2221 cggtggtgaa ggtcacaacc tgcaggagca ctccgtgatc ctgatccgtg gcggtcgtgt
     2281 taaagacctc ccgggtgttc gttaccacac cgtacgtggt gcgcttgact gctccggcgt
     2341 taaagaccgt aagcaggctc gttccaagta tggcgtgaag cgtcctaagg cttaatggtt
     2401 ctccaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     2461 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     2521 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat
     2581 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc
     2641 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc
     2701 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     2761 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     2821 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     2881 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     2941 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     3001 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3061 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3121 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3181 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3241 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     3301 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     3361 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     3421 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     3481 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     3541 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     3601 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagat
     3661 gtcagagatg gagttttgaa aggtgacgtg aagcataaac tgttgttgga aggaggtgga
     3721 catcatcgag tggactttaa gacaatctac agagctaaga aagctgtgaa attgccagac
     3781 tatcactttg tggatcaccg aattgaaatt ttgaaccatg ataaagatta caataaagtg
     3841 actgtgtatg agtcagcagt agctagaaac tcaactgatg gtatggacga gctctacgta
     3901 agcgcttaat aggtaccgcg gccgcgctga ggggctggcc gctcacaatt ccacacaaca
     3961 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4021 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4081 aatgaatcgg ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa ttctcatgtt
     4141 tgacagctta tcatcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag
     4201 caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac
     4261 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     4321 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     4381 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     4441 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     4501 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     4561 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     4621 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4681 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4741 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4801 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4861 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     4921 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     4981 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc
     5041 ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt
     5101 atttattcgc gataagctca tggagcggcg taaccgtcgc acaggaagga cagagaaagc
     5161 gcggatctgg gaagtgacgg acagaacggt caggacctgg attggggagg cggttgccgc
     5221 cgctgctgct gacggtgtga cgttctctgt tccggtcaca ccacatacgt tccgccattc
     5281 ctatgcgatg cacatgctgt atgccggtat accgctgaaa gttctgcaaa gcctgatggg
     5341 acataagtcc atcagttcaa cggaagtcta cacgaaggtt tttgcgctgg atgtggctgc
     5401 ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg gttgcgatgc tgaaacaatt
     5461 atcctgagaa taaatgcctt ggcctttata tggaaatgtg gaactgagtg gatatgctgt
     5521 ttttgtctgt taaacagaga agctggctgt tatccactga gaagcgaacg aaacagtcgg
     5581 gaaaatctcc cattatcgta gagatccgca ttattaatct caggagcctg tgtagcgttt
     5641 ataggaagta gtgttctgtc atgatgcctg caagcggtaa cgaaaacgat ttgaatatgc
     5701 cttcaggaac aatagaaatc ttcgtgcggt gttacgttga agtggagcgg attatgtcag
     5761 caatggacag aacaacctaa tgaacacaga accatgatgt ggtctgtcct tttacagcca
     5821 gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg gctggttgcc ctcgccgctg
     5881 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
     5941 gacaccgcgg ccggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     6001 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     6061 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     6121 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     6181 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     6241 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     6301 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     6361 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     6421 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggtc
     6481 gagtgagcga ggaagcacca gggaacagca cttatatatt ctgcttacac acgatgcctg
     6541 aaaaaacttc ccttggggtt atccacttat ccacggggat atttttataa ttattttttt
     6601 tatagttttt agatcttctt ttttagagcg ccttgtaggc ctttatccat gctggttcta
     6661 gagaaggtgt tgtgacaaat tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc
     6721 tgtctgtgac aaattgccct taaccctgtg acaaattgcc ctcagaagaa gctgtttttt
     6781 cacaaagtta tccctgctta ttgactcttt tttatttagt gtgacaatct aaaaacttgt
     6841 cacacttcac atggatctgt catggcggaa acagcggtta tcaatcacaa gaaacgtaaa
     6901 aatagcccgc gaatcgtcca gtcaaacgac ctcactgagg cggcatatag tctctcccgg
     6961 gatcaaaaac gtatgctgta tctgttcgtt gaccagatca gaaaatctga tggcacccta
     7021 caggaacatg acggtatctg cgagatccat gttgctaaat atgctgaaat attcggattg
     7081 acctctgcgg aagccagtaa ggatatacgg caggcattga agagtttcgc ggggaaggaa
     7141 gtggtttttt atcgccctga agaggatgcc ggcgatgaaa aaggctatga atcttttcct
     7201 tggtttatca aacgtgcgca cagtccatcc agagggcttt acagtgtaca tatcaaccca
     7261 tatctcattc ccttctttat cgggttacag aaccggttta cgcagtttcg gcttagtgaa
     7321 acaaaagaaa tcaccaatcc gtatgccatg cgtttatacg aatccctgtg tcagtatcgt
     7381 aagccggatg gctcaggcat cgtctctctg aaaatcgact ggatcataga gcgttaccag
     7441 ctgcctcaaa gttaccagcg tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt
     7501 aatgagatca acagcagaac tccaatgcgc ctctcataca ttgagaaaaa gaaaggccgc
     7561 cagacgactc atatcgtatt ttccttccgc gatatcactt ccatgacgac aggatagtct
     7621 gagggttatc tgtcacagat ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg
     7681 taatttgtca cagttttgct gtttccttca gcctgcatgg attttctcat actttttgaa
     7741 ctgtaatttt taaggaagcc aaatttgagg gcagtttgtc acagttgatt tccttctctt
     7801 tcccttcgtc atgtgacctg atatcggggg ttagttcgtc atcattgatg agggttgatt
     7861 atcacagttt attactctga attggctatc cgcgtgtgta cctctacctg gagtttttcc
     7921 cacggtggat atttcttctt gcgctgagcg taagagctat ctgacagaac agttcttctt
     7981 tgcttcctcg ccagttcgct cgctatgctc ggttacacgg ctgcggcgag cgctagtgat
     8041 aataagtgac tgaggtatgt gctcttctta tctccttttg tagtgttgct cttattttaa
     8101 acaactttgc ggttttttga tgactttgcg attttgttgt tgctttgcag taaattgcaa
     8161 gatttaataa aaaaacgcaa agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa
     8221 cacttaacca gtgcataaac gctggtcatg aaatgacgaa ggctatcgcc attgcacagt
     8281 ttaatgatga cagcccggaa gcgaggaaaa taacccggcg ctggagaata ggtgaagcag
     8341 cggatttagt tggggtttct tctcaggcta tcagagatgc cgagaaagca gggcgactac
     8401 cgcacccgga tatggaaatt cgaggacggg ttgagcaacg tgttggttat acaattgaac
     8461 aaattaatca tatgcgtgat gtgtttggta cgcgattgcg acgtgctgaa gacgtatttc
     8521 caccggtgat cggggttgct gcccataaag gtggcgttta caaaacctca gtttctgttc
     8581 atcttgctca ggatctggct ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc
     8641 cccagggaac agcctcaatg tatcacggat gggtaccaga tcttcatatt catgcagaag
     8701 acactctcct gcctttctat cttggggaaa aggacgatgt cacttatgca ataaagccca
     8761 cttgctggcc ggggcttgac attattcctt cctgtctggc tctgcaccgt attgaaactg
     8821 agttaatggg caaatttgat gaaggtaaac tgcccaccga tccacacctg atgctccgac
     8881 tggccattga aactgttgct catgactatg atgtcatagt tattgacagc gcgcctaacc
     8941 tgggtatcgg cacgattaat gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg
     9001 ctgagttgtt tgactacacc tccgcactgc agtttttcga tatgcttcgt gatctgctca
     9061 agaacgttga tcttaaaggg ttcgagcctg atgtacgtat tttgcttacc aaatacagca
     9121 atagtaatgg ctctcagtcc ccgtggatgg aggagcaaat tcgggatgcc tggggaagca
     9181 tggttctaaa aaatgttgta cgtgaaacgg atgaagttgg taaaggtcag atccggatga
     9241 gaactgtttt tgaacaggcc attgatcaac gctcttcaac tggtgcctgg agaaatgctc
     9301 tttctatttg ggaacctgtc tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg
     9361 agattagata atgaagcgtg cgcctgttat tccaaaacat acgctcaata ctcaaccggt
     9421 tgaagatact tcgttatcga caccagctgc cccgatggtg gattcgttaa ttgcgcgcgt
     9481 aggagtaatg gctcgcggta atgccattac tttgcctgta tgtggtcggg atgtgaagtt
     9541 tactcttgaa gtgctccggg gtgatagtgt tgagaagacc tctcgggtat ggtcaggtaa
     9601 tgaacgtgac caggagctgc ttactgagga cgcactggat gatctcatcc cttcttttct
     9661 actgactggt caacagacac cggcgttcgg tcgaagagta tctggtgtca tagaaattgc
     9721 cgatgggagt cgccgtcgta aagctgctgc acttaccgaa agtgattatc gtgttctggt
     9781 tggcgagctg gatgatgagc agatggctgc attatccaga ttgggtaacg attatcgccc
     9841 aacaagtgct tatgaacgtg gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg
     9901 aaatatttct gcgctggctg atgcggaaaa tatttcacgt aagattatta cccgctgtat
     9961 caacaccgcc aaattgccta aatcagttgt tgctcttttt tctcaccccg gtgaactatc
    10021 tgcccggtca ggtgatgcac ttcaaaaagc ctttacagat aaagaggaat tacttaagca
    10081 gcaggcatct aaccttcatg agcagaaaaa agctggggtg atatttgaag ctgaagaagt
    10141 tatcactctt ttaacttctg tgcttaaaac gtcatctgca tcaagaacta gtttaagctc
    10201 acgacatcag tttgctcctg gagcgacagt attgtataag ggcgataaaa tggtgcttaa
    10261 cctggacagg tctcgtgttc caactgagtg tatagagaaa attgaggcca ttcttaagga
    10321 acttgaaaag ccagcaccct gatgcgacca cgttttagtc tacgtttatc tgtctttact
    10381 taatgtcctt tgttacaggc cagaaagcat aactggcctg aatattctct ctgggcccac
    10441 tgttccactt gtatcgtcgg tctgataatc agactgggac cacggtccca ctcgtatcgt
    10501 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
    10561 tgggaccacg gtcccactcg tatcgtcggt ctgataatca gactgggacc acggtcccac
    10621 tcgtatcgtc ggtctgatta ttagtctggg accatggtcc cactcgtatc gtcggtctga
    10681 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag tctggaacca
    10741 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
    10801 tcggtctgat tattagtctg ggaccacgat cccactcgtg ttgtcggtct gattatcggt
    10861 ctgggaccac ggtcccactt gtattgtcga tcagactatc agcgtgagac tacgattcca
    10921 tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa cctgt
//
