LOCUS       sequence-57007-e        9291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH090
FEATURES             Location/Qualifiers
     source          1..9291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2104..2127
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2143..2166
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2182..2226
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        complement(join(2282..2288,2289..2306,2307..2312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2327..2348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2568..3227)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3142..3161
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3228..3330)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4188..4802
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4279..4810
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4878..5097
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             5188..5943
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5946..6196
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6522..7697
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7697..8668
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    8741..9214
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaaac catggccgcc tcaaaaggag aagaaacgac aatgggtgtt
     1381 atcaagccag acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc
     1441 gtcattgagg gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa
     1501 gtgaaggagg gtgccccact tccattctct tatgatatcc ttactacagc cttcgcatat
     1561 ggaaatcgag cattcacaaa atacccagac gacattccaa attacttcaa gcagtcattt
     1621 ccagagggat attcttggga gagaacaatg acattcgagg acaagggtat agttaaagtg
     1681 aaatctgata ttagtatgga agaagattca ttcatctatg agatacacct gaagggcgag
     1741 aactttcctc cgaatggacc agtgatgcag aaaaagacaa caggatggga cgcaagtact
     1801 gaacgtatgt atgtcagaga tggagttttg aaaggtgacg tgaagcataa actgttgttg
     1861 gaaggaggtg gacatcatcg agtggacttt aagacaatct acagagctaa gaaagctgtg
     1921 aaattgccag actatcactt tgtggatcac cgaattgaaa ttttgaacca tgataaagat
     1981 tacaataaag tgactgtgta tgagtcagca gtagctagaa actcaactga tggtatggac
     2041 gagctctacg tatccggagg tggtggtagt ggaggaggcg gatcgggagg aggaggttcg
     2101 cgatggtcac atccacaatt tgagaaagga ggtggaggat cattggaagt tctcttccaa
     2161 ggacctggag gaggtggtag taaagagaca gcggctgcaa aattcgagag acagcatatg
     2221 gactcatggg cttaataggt accgcggccg cgctgagggg ctggccgctc acaattccac
     2281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2401 tgcattaatg aatcggccaa cgcgaacccc ttgcggccgc ccgggccgtc gaccaattct
     2461 catgtttgac agcttatcat cgaatttctg ccattcatcc gcttattatc acttattcag
     2521 gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     2581 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2641 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2701 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2761 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2821 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2881 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2941 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     3001 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     3061 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     3121 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     3181 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3241 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     3301 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag
     3361 ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc
     3421 acaggtattt attcgcgata agctcatgga gcggcgtaac cgtcgcacag gaaggacaga
     3481 gaaagcgcgg atctgggaag tgacggacag aacggtcagg acctggattg gggaggcggt
     3541 tgccgccgct gctgctgacg gtgtgacgtt ctctgttccg gtcacaccac atacgttccg
     3601 ccattcctat gcgatgcaca tgctgtatgc cggtataccg ctgaaagttc tgcaaagcct
     3661 gatgggacat aagtccatca gttcaacgga agtctacacg aaggtttttg cgctggatgt
     3721 ggctgcccgg caccgggtgc agtttgcgat gccggagtct gatgcggttg cgatgctgaa
     3781 acaattatcc tgagaataaa tgccttggcc tttatatgga aatgtggaac tgagtggata
     3841 tgctgttttt gtctgttaaa cagagaagct ggctgttatc cactgagaag cgaacgaaac
     3901 agtcgggaaa atctcccatt atcgtagaga tccgcattat taatctcagg agcctgtgta
     3961 gcgtttatag gaagtagtgt tctgtcatga tgcctgcaag cggtaacgaa aacgatttga
     4021 atatgccttc aggaacaata gaaatcttcg tgcggtgtta cgttgaagtg gagcggatta
     4081 tgtcagcaat ggacagaaca acctaatgaa cacagaacca tgatgtggtc tgtcctttta
     4141 cagccagtag tgctcgccgc agtcgagcga cagggcgaag ccctcggctg gttgccctcg
     4201 ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt
     4261 gtgcgagaca ccgcggccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
     4321 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
     4381 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
     4441 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
     4501 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
     4561 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
     4621 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
     4681 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
     4741 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
     4801 ccggtcgagt gagcgaggaa gcaccaggga acagcactta tatattctgc ttacacacga
     4861 tgcctgaaaa aacttccctt ggggttatcc acttatccac ggggatattt ttataattat
     4921 tttttttata gtttttagat cttctttttt agagcgcctt gtaggccttt atccatgctg
     4981 gttctagaga aggtgttgtg acaaattgcc ctttcagtgt gacaaatcac cctcaaatga
     5041 cagtcctgtc tgtgacaaat tgcccttaac cctgtgacaa attgccctca gaagaagctg
     5101 ttttttcaca aagttatccc tgcttattga ctctttttta tttagtgtga caatctaaaa
     5161 acttgtcaca cttcacatgg atctgtcatg gcggaaacag cggttatcaa tcacaagaaa
     5221 cgtaaaaata gcccgcgaat cgtccagtca aacgacctca ctgaggcggc atatagtctc
     5281 tcccgggatc aaaaacgtat gctgtatctg ttcgttgacc agatcagaaa atctgatggc
     5341 accctacagg aacatgacgg tatctgcgag atccatgttg ctaaatatgc tgaaatattc
     5401 ggattgacct ctgcggaagc cagtaaggat atacggcagg cattgaagag tttcgcgggg
     5461 aaggaagtgg ttttttatcg ccctgaagag gatgccggcg atgaaaaagg ctatgaatct
     5521 tttccttggt ttatcaaacg tgcgcacagt ccatccagag ggctttacag tgtacatatc
     5581 aacccatatc tcattccctt ctttatcggg ttacagaacc ggtttacgca gtttcggctt
     5641 agtgaaacaa aagaaatcac caatccgtat gccatgcgtt tatacgaatc cctgtgtcag
     5701 tatcgtaagc cggatggctc aggcatcgtc tctctgaaaa tcgactggat catagagcgt
     5761 taccagctgc ctcaaagtta ccagcgtatg cctgacttcc gccgccgctt cctgcaggtc
     5821 tgtgttaatg agatcaacag cagaactcca atgcgcctct catacattga gaaaaagaaa
     5881 ggccgccaga cgactcatat cgtattttcc ttccgcgata tcacttccat gacgacagga
     5941 tagtctgagg gttatctgtc acagatttga gggtggttcg tcacatttgt tctgacctac
     6001 tgagggtaat ttgtcacagt tttgctgttt ccttcagcct gcatggattt tctcatactt
     6061 tttgaactgt aatttttaag gaagccaaat ttgagggcag tttgtcacag ttgatttcct
     6121 tctctttccc ttcgtcatgt gacctgatat cgggggttag ttcgtcatca ttgatgaggg
     6181 ttgattatca cagtttatta ctctgaattg gctatccgcg tgtgtacctc tacctggagt
     6241 ttttcccacg gtggatattt cttcttgcgc tgagcgtaag agctatctga cagaacagtt
     6301 cttctttgct tcctcgccag ttcgctcgct atgctcggtt acacggctgc ggcgagcgct
     6361 agtgataata agtgactgag gtatgtgctc ttcttatctc cttttgtagt gttgctctta
     6421 ttttaaacaa ctttgcggtt ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa
     6481 ttgcaagatt taataaaaaa acgcaaagca atgattaaag gatgttcaga atgaaactca
     6541 tggaaacact taaccagtgc ataaacgctg gtcatgaaat gacgaaggct atcgccattg
     6601 cacagtttaa tgatgacagc ccggaagcga ggaaaataac ccggcgctgg agaataggtg
     6661 aagcagcgga tttagttggg gtttcttctc aggctatcag agatgccgag aaagcagggc
     6721 gactaccgca cccggatatg gaaattcgag gacgggttga gcaacgtgtt ggttatacaa
     6781 ttgaacaaat taatcatatg cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg
     6841 tatttccacc ggtgatcggg gttgctgccc ataaaggtgg cgtttacaaa acctcagttt
     6901 ctgttcatct tgctcaggat ctggctctga aggggctacg tgttttgctc gtggaaggta
     6961 acgaccccca gggaacagcc tcaatgtatc acggatgggt accagatctt catattcatg
     7021 cagaagacac tctcctgcct ttctatcttg gggaaaagga cgatgtcact tatgcaataa
     7081 agcccacttg ctggccgggg cttgacatta ttccttcctg tctggctctg caccgtattg
     7141 aaactgagtt aatgggcaaa tttgatgaag gtaaactgcc caccgatcca cacctgatgc
     7201 tccgactggc cattgaaact gttgctcatg actatgatgt catagttatt gacagcgcgc
     7261 ctaacctggg tatcggcacg attaatgtcg tatgtgctgc tgatgtgctg attgttccca
     7321 cgcctgctga gttgtttgac tacacctccg cactgcagtt tttcgatatg cttcgtgatc
     7381 tgctcaagaa cgttgatctt aaagggttcg agcctgatgt acgtattttg cttaccaaat
     7441 acagcaatag taatggctct cagtccccgt ggatggagga gcaaattcgg gatgcctggg
     7501 gaagcatggt tctaaaaaat gttgtacgtg aaacggatga agttggtaaa ggtcagatcc
     7561 ggatgagaac tgtttttgaa caggccattg atcaacgctc ttcaactggt gcctggagaa
     7621 atgctctttc tatttgggaa cctgtctgca atgaaatttt cgatcgtctg attaaaccac
     7681 gctgggagat tagataatga agcgtgcgcc tgttattcca aaacatacgc tcaatactca
     7741 accggttgaa gatacttcgt tatcgacacc agctgccccg atggtggatt cgttaattgc
     7801 gcgcgtagga gtaatggctc gcggtaatgc cattactttg cctgtatgtg gtcgggatgt
     7861 gaagtttact cttgaagtgc tccggggtga tagtgttgag aagacctctc gggtatggtc
     7921 aggtaatgaa cgtgaccagg agctgcttac tgaggacgca ctggatgatc tcatcccttc
     7981 ttttctactg actggtcaac agacaccggc gttcggtcga agagtatctg gtgtcataga
     8041 aattgccgat gggagtcgcc gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt
     8101 tctggttggc gagctggatg atgagcagat ggctgcatta tccagattgg gtaacgatta
     8161 tcgcccaaca agtgcttatg aacgtggtca gcgttatgca agccgattgc agaatgaatt
     8221 tgctggaaat atttctgcgc tggctgatgc ggaaaatatt tcacgtaaga ttattacccg
     8281 ctgtatcaac accgccaaat tgcctaaatc agttgttgct cttttttctc accccggtga
     8341 actatctgcc cggtcaggtg atgcacttca aaaagccttt acagataaag aggaattact
     8401 taagcagcag gcatctaacc ttcatgagca gaaaaaagct ggggtgatat ttgaagctga
     8461 agaagttatc actcttttaa cttctgtgct taaaacgtca tctgcatcaa gaactagttt
     8521 aagctcacga catcagtttg ctcctggagc gacagtattg tataagggcg ataaaatggt
     8581 gcttaacctg gacaggtctc gtgttccaac tgagtgtata gagaaaattg aggccattct
     8641 taaggaactt gaaaagccag caccctgatg cgaccacgtt ttagtctacg tttatctgtc
     8701 tttacttaat gtcctttgtt acaggccaga aagcataact ggcctgaata ttctctctgg
     8761 gcccactgtt ccacttgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg
     8821 tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta
     8881 ttagtctggg accacggtcc cactcgtatc gtcggtctga taatcagact gggaccacgg
     8941 tcccactcgt atcgtcggtc tgattattag tctgggacca tggtcccact cgtatcgtcg
     9001 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg
     9061 gaaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc
     9121 gtatcgtcgg tctgattatt agtctgggac cacgatccca ctcgtgttgt cggtctgatt
     9181 atcggtctgg gaccacggtc ccacttgtat tgtcgatcag actatcagcg tgagactacg
     9241 attccatcaa tgcctgtcaa gggcaagtat tgacatgtcg tcgtaacctg t
//
