LOCUS       sequence-57008-e       10959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH094
FEATURES             Location/Qualifiers
     source          1..10959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2245..3435
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2597..2616)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             3772..3795
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3811..3834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3850..3894
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        complement(join(3950..3956,3957..3974,3975..3980))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3995..4016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4236..4895)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4810..4829
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4896..4998)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5856..6470
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5947..6478
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6546..6765
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6856..7611
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7614..7864
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8190..9365
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9365..10336
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10409..10882
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaaac catggccgcc tcaaaaggag aagaaacgac aatgggtgtt
     1381 atcaagccag acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc
     1441 gtcattgagg gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa
     1501 gtgaaggagg gtgccccact tccattctct tatgatatcc ttactacagc cttcgcatat
     1561 ggaaatcgag cattcacaaa atacccagac ggctgtcgag atatgacggt gttcacaaag
     1621 ttccttaaat tttacttttg gttacatatt ttttcttttt gaaaccaaat ctttatcttt
     1681 gtagcacttt cacggtagcg aaacgttagt ttgaatggaa agatgcctgc agacacataa
     1741 agacaccaaa ctctcatcaa tagttccgta aatttttatt gacagaactt attgacggca
     1801 gtggcaggtg tcataaaaaa aaccatgagg gtaataaata atggcaacag ttaaccagct
     1861 ggtacgcaaa ccacgtgctc gcaaagttgc gaaaagcaac gtgcctgcgc tggaagcatg
     1921 cccgcaaaaa cgtggcgtat gtactcgtgt atatactacc actcctaaaa aaccgaactc
     1981 cgcgctgcgt aaagtatgcc gtgttcgtct gactaacggt ttcgaagtga cttcctacat
     2041 cggtggtgaa ggtcacaacc tgcaggagca ctccgtgatc ctgatccgtg gcggtcgtgt
     2101 taaagacctc ccgggtgttc gttaccacac cgtacgtggt gcgcttgact gctccggcgt
     2161 taaagaccgt aagcaggctc gttccaagta tggcgtgaag cgtcctaagg cttaatggtt
     2221 ctccaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     2281 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     2341 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat
     2401 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc
     2461 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc
     2521 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     2581 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     2641 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     2701 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     2761 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     2821 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     2881 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2941 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3001 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3061 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     3121 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     3181 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     3241 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     3301 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     3361 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     3421 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagat
     3481 gtcagagatg gagttttgaa aggtgacgtg aagcataaac tgttgttgga aggaggtgga
     3541 catcatcgag tggactttaa gacaatctac agagctaaga aagctgtgaa attgccagac
     3601 tatcactttg tggatcaccg aattgaaatt ttgaaccatg ataaagatta caataaagtg
     3661 actgtgtatg agtcagcagt agctagaaac tcaactgatg gtatggacga gctctacgta
     3721 tccggaggtg gtggtagtgg aggaggcgga tcgggaggag gaggttcgcg atggtcacat
     3781 ccacaatttg agaaaggagg tggaggatca ttggaagttc tcttccaagg acctggagga
     3841 ggtggtagta aagagacagc ggctgcaaaa ttcgagagac agcatatgga ctcatgggct
     3901 taataggtac cgcggccgcg ctgaggggct ggccgctcac aattccacac aacatacgag
     3961 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4081 tcggccaacg cgaacccctt gcggccgccc gggccgtcga ccaattctca tgtttgacag
     4141 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
     4201 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     4261 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     4321 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     4381 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     4441 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     4501 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     4561 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     4621 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     4681 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     4741 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     4801 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     4861 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     4921 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     4981 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     5041 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     5101 tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat
     5161 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
     5221 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc
     5281 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa
     5341 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca
     5401 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg
     5461 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt
     5521 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat
     5581 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga
     5641 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag
     5701 gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg
     5761 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg
     5821 ctcgccgcag tcgagcgaca gggcgaagcc ctcggctggt tgccctcgcc gctgggctgg
     5881 cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc
     5941 gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     6001 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     6061 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     6121 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     6181 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     6241 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     6301 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     6361 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     6421 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggtcgagtga
     6481 gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa
     6541 cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt
     6601 ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag
     6661 gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg
     6721 tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa
     6781 gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact
     6841 tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc
     6901 ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa
     6961 aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa
     7021 catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct
     7081 gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt
     7141 ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt
     7201 atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc
     7261 attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa
     7321 gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg
     7381 gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct
     7441 caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag
     7501 atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg
     7561 actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt
     7621 tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt
     7681 gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa
     7741 tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt
     7801 cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca
     7861 gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt
     7921 ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc
     7981 ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag
     8041 tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact
     8101 ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta
     8161 ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta
     8221 accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg
     8281 atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt
     8341 tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc
     8401 cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta
     8461 atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg
     8521 tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg
     8581 ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg
     8641 gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc
     8701 tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct
     8761 ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa
     8821 tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca
     8881 ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta
     8941 tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt
     9001 tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg
     9061 ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta
     9121 atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc
     9181 taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg
     9241 tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta
     9301 tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta
     9361 gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga
     9421 tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt
     9481 aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct
     9541 tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg
     9601 tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac
     9661 tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg
     9721 gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga
     9781 gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag
     9841 tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat
     9901 ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac
     9961 cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg
    10021 gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc
    10081 atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac
    10141 tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca
    10201 tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga
    10261 caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga
    10321 aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt
    10381 cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc
    10441 acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct
    10501 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
    10561 cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat
    10621 cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta
    10681 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc
    10741 cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc
    10801 tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga
    10861 ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg
    10921 cctgtcaagg gcaagtattg acatgtcgtc gtaacctgt
//
