LOCUS       sequence-57009-e        5026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for GI-ZFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     Light-inducible spatiotemporal control of gene activation by 
            customizable zinc finger transcription factors.
  JOURNAL   J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. 
            Epub 2012 Sep 27.
  PUBMED    22963237
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja3065667";
            journalName: "J Am Chem Soc"; date: "2012-10-10- 10"; volume: "134";
            issue: "40"; pages: "16480-3"
COMMENT     Sequence Label: pGL3-Basic-9xSeq2-Luc
FEATURES             Location/Qualifiers
     source          1..5026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        201..239
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     236..260
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             296..1948
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(362..380)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1989..2110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2000..2019)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2054..2073
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2358..2375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2529..3117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2609..2628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3288..4079,4080..4148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3911..3930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4149..4253)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4280..4735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4367..4386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4577..4598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4866..4914
                     /note="synthetic polyadenylation signal"
     misc_feature    4928..5019
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4968..4987
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagaaa actgcaaaag gtcgagaaac
       61 tgcaaaaggt cgagaaactg caaaaggtcg agaaactgca aaaggtcgag aaactgcaaa
      121 aggtcgagaa actgcaaaag gtcgagaaac tgcaaaaggt cgagaaactg caaaaggtcg
      181 agaaactgca aaagagatct ggtaggcgtg tacggtggga ggtctatata agcagagctc
      241 gtttagtgaa ccgtcagatc aagcttggca ttccggtact gttggtaaag ccaccatgga
      301 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      361 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      421 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      481 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      541 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      601 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      661 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
      721 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
      781 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
      841 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
      901 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
      961 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     1021 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     1081 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     1141 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     1201 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     1261 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     1321 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     1381 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     1441 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     1501 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     1561 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     1621 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     1681 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     1741 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     1801 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     1861 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     1921 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
     1981 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2041 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2101 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2161 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
     2221 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     2281 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2341 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4261 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4321 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4381 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4441 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4501 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4561 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4621 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4681 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat
     4741 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4801 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
     4861 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     4921 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     4981 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata
//
