LOCUS       sequence-57010-e        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GI-ZFP2 for light-inducible activation of gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Polstein LR, Gersbach CA
  TITLE     Light-inducible spatiotemporal control of gene activation by 
            customizable zinc finger transcription factors.
  JOURNAL   J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. 
            Epub 2012 Sep 27.
  PUBMED    22963237
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja3065667";
            journalName: "J Am Chem Soc"; date: "2012-10-10- 10"; volume: "134";
            issue: "40"; pages: "16480-3"
COMMENT     Sequence Label: pcDNA3-GI-ZFP2
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      944..953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4478..4501
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4508..4528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4902..4920)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4903..4920)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4940..4957)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4946..5170
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5216..5644
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5303..5322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5513..5534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5653..5673)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5658..5988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5839..5974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5901..5920
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6055..6849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6109..6128)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6719..6738
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7023..7144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7060..7079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7114..7133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7193..7209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7193..7209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7206..7228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7217..7233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7241..7247,7248..7265,7266..7271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7286..7307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7424..7441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7595..8183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7675..7694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8354..9145,9146..9214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8977..8996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9215..9276,1..2))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggctagttc
      961 atcttcatct gagagatgga tcgatggtct tcagttctct tccttgttat ggcctccgcc
     1021 acgagatcct caacaacata aggatcaagt cgttgcttat gttgaatatt ttggtcaatt
     1081 tacatcagag caattcccag atgacattgc tgagttggtc cggcatcagt atccatcaac
     1141 cgagaagcga cttttggacg atgtgctggc gatgtttgtc cttcatcatc cggagcatgg
     1201 tcatgcagtc attcttccaa tcatttcatg tcttattgat ggctcgttgg tgtacagcaa
     1261 ggaagctcat ccgtttgcct ctttcatatc tttagtttgc ccaagtagtg agaatgacta
     1321 ttcggagcaa tgggctttgg catgtggaga aatccttcgc attttgactc attacaaccg
     1381 tcccatttat aaaactgagc agcaaaatgg agatacagag agaaattgtc tgagcaaagc
     1441 tacaactagt ggttctccga cttcagagcc taaggctgga tcaccaacac agcatgaaag
     1501 gaaaccttta aggcctttgt ctccatggat cagtgatata ctacttgctg ctcctcttgg
     1561 tataagaagt gactatttcc gatggtgtag tggtgtaatg ggtaaatatg ctgctggaga
     1621 gctcaagccg ccaaccattg cttctcgagg atctggtaaa catcctcaac tgatgccttc
     1681 aaccccaaga tgggctgttg ctaatggagc tggtgtcata ctgagtgttt gtgatgatga
     1741 agttgctcga tatgagactg ctacgctgac agcggtcgct gtccctgcac ttcttcttcc
     1801 tccgccaacg acatccttag atgagcatct agttgctggc cttccagctc ttgaaccata
     1861 tgcacgtttg tttcatagat actatgccat tgcaactcca agtgctacgc agagacttct
     1921 tcttggactc ttagaagcac caccgtcgtg ggctccagat gcacttgatg ctgctgtaca
     1981 gcttgtggaa ctccttcgag ctgctgaaga ttatgcatct ggtgtaaggc tacccaggaa
     2041 ctggatgcat ttgcacttct tgcgggctat aggaattgct atgtctatga gggcaggtgt
     2101 tgctgctgat gctgcagccg ctttgctttt ccgcatactc tcacagccgg cactgctttt
     2161 tcctccgcta agtcaagttg agggagtaga aattcagcac gcgcctattg gtggctacag
     2221 ttcaaattac agaaaacaga tagaagttcc tgcagcagaa gcaaccattg aagccactgc
     2281 ccaaggaatt gcctcaatgc tttgtgctca tggtcctgaa gttgagtgga gaatttgcac
     2341 tatatgggaa gctgcttatg gtttgatccc tttaaattct tcggcggttg atcttcccga
     2401 aatcatagtt gctaccccac tgcaacctcc tatcttgtca tggaatttat acattccact
     2461 cctcaaagta cttgaatatc ttccacgggg gagtccttcg gaagcatgct tgatgaaaat
     2521 atttgttgcc actgtggaaa caatactcag tagaactttt ccgcctgaat cttccaggga
     2581 actaaccaga aaagctagat cgagttttac cacaagatca gcgaccaaaa atcttgctat
     2641 gtctgagctt cgtgctatgg tccatgctct ctttttagaa tcatgcgctg gtgtggaatt
     2701 agcttcacgc ctactttttg ttgtgttgac tgtatgtgtt agccatgaag cacagtctag
     2761 tggtagcaag agaccgagaa gtgaatatgc tagtactact gaaaatattg aggcgaatca
     2821 acctgtatct aacaatcaaa ctgctaaccg taaaagtagg aatgtcaagg gacagggacc
     2881 tgtggcagca tttgattcat acgttcttgc tgctgtttgt gctcttgcct gtgaggttca
     2941 gctgtatcct atgatctctg gtggggggaa cttttccaat tctgccgtgg ctggaactat
     3001 tacaaagcct gtaaagataa atgggtcatc taaagagtat ggagctggga ttgactcggc
     3061 aattagtcat acgcgccgaa ttttggcaat cctagaggca ctcttttcat taaaaccatc
     3121 ttctgtgggg actccatgga gttacagttc tagtgagata gttgctgcgg ccatggttgc
     3181 agctcatatt tccgaactgt tcagacgttc aaaggccttg acgcatgcat tgtctgggtt
     3241 gatgagatgt aagtgggata aggaaattca taaaagagca tcatcattat ataacctcat
     3301 agatgttcac agcaaagttg ttgcctccat tgttgacaaa gctgaaccct tggaagccta
     3361 ccttaagaat acaccggttc agaaggattc tgtgacctgt ttaaactgga aacaagagaa
     3421 cacatgtgca agcaccacat gctttgatac agcggtgaca tccgcctcaa ggactgaaat
     3481 gaatccaaga ggaaaccata agtatgctag acattcagat gaaggctcag gaagaccctc
     3541 agagaagggt atcaaagatt tcctcttgga tgcttctgat ctagcgaatt tcctcacagc
     3601 tgatagactc gcagggttct attgtggtac acaaaagctt ttgaggtcag tgcttgcaga
     3661 gaaaccggag ctgtctttct ccgttgtttc actgttatgg cacaaactga ttgctgctcc
     3721 tgaaatccag cccaccgcag aaagcacctc tgcgcaacaa ggatggagac aggttgttga
     3781 tgcgctatgc aatgtcgtat ctgcaacgcc agcgaaagca gcagcagcag ttgtccttca
     3841 ggctgaaagg gagttgcagc cttggatcgc caaagatgat gaagaaggcc aaaaaatgtg
     3901 gaaaatcaac caacggatag tcaaagtgtt ggtggaactc atgcgcaatc atgacaggcc
     3961 tgagtcactg gtgattctcg caagtgcatc agatcttctt ctgcgggcaa ctgatggaat
     4021 gcttgttgat ggagaagctt gtacattacc tcaacttgag ctacttgaag ccacggcaag
     4081 agcaatacag ccggtgctag cttgggggcc atctggacta gcagtggtcg acggtttatc
     4141 caatctattg aagtgtcgtc taccagcaac aatacggtgc ctttcacacc caagtgcaca
     4201 cgtacgtgcc ttaagcacgt cagtactacg tgatatcatg aaccaaagct ccatacccat
     4261 caaagtaact ccaaaactgc caacaacaga gaagaacgga atgaatagtc cgtcctatcg
     4321 attcttcaac gccgcctcaa tagactggaa agccgatatc caaaactgtt taaactggga
     4381 agctcacagc ttgctctcca caactatgcc tactcagttt ctcgacactg cggctcggga
     4441 actcggctgt actatatcct tgtcccaagc ggccgccgat tacaaggatg acgatgacaa
     4501 gatggccccc aagaagaaga ggaaggtggg ccgcctcgag cccggagaga ggcctttcca
     4561 gtgtagaatt tgcatgagaa attttagtag gagcgataat ttgagcgttc acatcaggac
     4621 gcataccggg gaaaagccgt ttgcctgcga catctgtggt cggaaattcg cacagaagat
     4681 taacctgcaa gtccacacta aaatccacac aggcgaaaag ccctttcagt gccggatatg
     4741 tatgcgaaac ttttcaagat ctgacgtgct gtccgaacac atccgcacac ataccggcga
     4801 gaaaccattc gcttgtgata tatgcgggcg caagttcgcc cagcggaacc accgaaccac
     4861 tcatacaaag attcatcttc gcgtgatcta gagggcccta ttctatagtg tcacctaaat
     4921 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4981 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5041 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5101 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5161 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5281 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5341 gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct
     5401 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5521 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5581 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5641 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca
     5701 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5821 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5881 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5941 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6001 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6061 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6121 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6181 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6241 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6301 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6361 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6421 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6481 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6541 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6601 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6661 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6721 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6781 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6841 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6901 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6961 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7021 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7141 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     7801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9241 gcatttatca gggttattgt ctcatgagcg gataca
//
