LOCUS       sequence-57012-e        5450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Calmann MA, Marinus MG
  TITLE     Regulated expression of the Escherichia coli dam gene.
  JOURNAL   J Bacteriol. 2003 Aug;185(16):5012-4.
  PUBMED    12897023
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2003-08"; volume: "185(16)"; pages: "5012-4"
COMMENT     Sequence Label: pMQ430
FEATURES             Location/Qualifiers
     source          1..5450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     207..226
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(1231..1248)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1401..1487
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1579..1606
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1625..1716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1717..1785,1786..2577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1935..1954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2619..3074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2706..2725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      3185..3773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3674..3693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3927..3944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3959..4099
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4025..4044)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4185..4207
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4346..4365
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4545..5423)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(5450,1..284)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
       61 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa
      121 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga
      181 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga
      241 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc
      301 tagcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgaagaaa
      361 aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgataa tattaaacgg
      421 catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc ggtgtttctc
      481 aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat cagtctctat
      541 aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct gtttgttccc
      601 gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa aagccaggat
      661 ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa cggcctgtgt
      721 cgttacaatc tgcgcggtga gtttaacgtg ccgttcggcc gctgcaaaaa accctatttc
      781 ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt ctattgtgag
      841 tcttacggcg atagcatggc gcgcgcagat gatgcatccg tcgtctattg cgatccgcct
      901 tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag ttttacgctt
      961 gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg
     1021 ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat
     1081 gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa ggtggacgaa
     1141 ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt ctaagcttgg
     1201 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
     1261 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
     1321 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
     1381 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1441 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     1501 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     1561 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
     1621 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1681 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1741 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1801 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1861 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1921 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     1981 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2041 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2101 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     2161 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2221 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     2281 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2341 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2401 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2461 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2521 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2581 tcagaccaag tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt
     2641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2761 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2821 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaacttgaac
     2941 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     3001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3061 aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3301 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3841 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3901 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3961 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     4021 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     4081 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4141 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4201 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag caaggagatg gcgcccaaca
     4261 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     4321 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     4381 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga
     4441 cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc
     4501 ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
     4561 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
     4621 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
     4681 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
     4741 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
     4801 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
     4861 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
     4921 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc
     4981 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
     5041 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     5101 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     5161 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     5221 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     5281 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     5341 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     5401 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
//
