LOCUS       sequence-57016-e        5733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse Nanog.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-Nanog
FEATURES             Location/Qualifiers
     source          1..5733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(674..1465,1466..1534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1297..1316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1535..1639)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1669..2262
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2325..2682
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2741..3157
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3087..3109
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3125..3141
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3167..3541
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3479..3495
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    3592..3616
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4545..4569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             4886..5355
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5484..5501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5655..5733,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa nggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
      661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc
     1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
     2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
     2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
     2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
     2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata
     2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
     2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
     2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt
     3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
     3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
     3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
     3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
     3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
     3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact
     3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaaat cacaagtttg
     3601 tacaaaaaag caggctctga catgagtgtg ggtcttcctg gtccccacag tttgcctagt
     3661 tctgaggaag catcgaattc tgggaacgcc tcatcaatgc ctgcagtttt tcatcccgag
     3721 aactattctt gcttacaagg gtctgctact gagatgctct gcacagaggc tgcctctcct
     3781 cgcccttcct ctgaagacct gcctcttcaa ggcagccctg attcttctac cagtcccaaa
     3841 caaaagctct caagtcctga ggctgacaag ggccctgagg aggaggagaa caaggtcctt
     3901 gccaggaagc agaagatgcg gactgtgttc tctcaggccc agctgtgtgc actcaaggac
     3961 aggtttcaga agcagaagta cctcagcctc cagcagatgc aagaactctc ctccattctg
     4021 aacctgagct ataagcaggt taagacctgg tttcaaaacc aaaggatgaa gtgcaagcgg
     4081 tggcagaaaa accagtggtt gaagactagc aatggtctga ttcagaaggg ctcagcacca
     4141 gtggagtatc ccagcatcca ttgcagctat ccccagggct atctggtgaa cgcatctgga
     4201 agcctttcca tgtggggcag ccagacttgg accaacccaa cttggagcag ccagacctgg
     4261 accaacccaa cttggaacaa ccagacctgg accaacccaa cttggagcag ccaggcctgg
     4321 accgctcagt cctggaacgg ccagccttgg aatgctgctc cgctccataa cttcggggag
     4381 gactttctgc agccttacgt acagttgcag caaaacttct ctgccagtga tttggaggtg
     4441 aatttggaag ccactaggga aagccatgcg cattttagca ccccacaagc cttggaatta
     4501 ttcctgaact actctgtgac tccaccaggt gaaatatgag acttacccag ctttcttgta
     4561 caaagtggtg atattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca
     4621 aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat
     4681 gtactattta aactcgagcg gccgccagca cagtggtcga cgataaaata aaagatttta
     4741 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     4801 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     4861 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     4921 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     4981 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     5041 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     5101 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5161 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     5221 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     5281 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5341 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt cttaagtatt
     5401 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     5461 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5701 tcaagtcaga ggtggcgaaa cccgacagga cta
//
