LOCUS       sequence-57021-e        5856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human OCT3/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     Sequence Label: pMXs-hOCT3/4
FEATURES             Location/Qualifiers
     source          1..5856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(674..1465,1466..1534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1297..1316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1535..1639)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1669..2262
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2325..2682
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2741..3157
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3087..3109
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3125..3141
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3167..3541
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3479..3495
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             5009..5478
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5607..5624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5778..5856,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
       61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
      661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc
     1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
     2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
     2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
     2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
     2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
     2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata
     2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
     2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
     2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
     2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
     2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
     2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
     2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
     3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
     3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
     3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt
     3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
     3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
     3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
     3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
     3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
     3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact
     3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaatt cgcccttcac
     3601 catggcggga cacctggctt cggatttcgc cttctcgccc cctccaggtg gtggaggtga
     3661 tgggccaggg gggccggagc cgggctgggt tgatcctcgg acctggctaa gcttccaagg
     3721 ccctcctgga gggccaggaa tcgggccggg ggttgggcca ggctctgagg tgtgggggat
     3781 tcccccatgc cccccgccgt atgagttctg tggggggatg gcgtactgtg ggccccaggt
     3841 tggagtgggg ctagtgcccc aaggcggctt ggagacctct cagcctgagg gcgaagcagg
     3901 agtcggggtg gagagcaact ccgatggggc ctccccggag ccctgcaccg tcacccctgg
     3961 tgccgtgaag ctggagaagg agaagctgga gcaaaacccg gaggagtccc aggacatcaa
     4021 agctctgcag aaagaactcg agcaatttgc caagctcctg aagcagaaga ggatcaccct
     4081 gggatataca caggccgatg tggggctcac cctgggggtt ctatttggga aggtattcag
     4141 ccaaacgacc atctgccgct ttgaggctct gcagcttagc ttcaagaaca tgtgtaagct
     4201 gcggcccttg ctgcagaagt gggtggagga agctgacaac aatgaaaatc ttcaggagat
     4261 atgcaaagca gaaaccctcg tgcaggcccg aaagagaaag cgaaccagta tcgagaaccg
     4321 agtgagaggc aacctggaga atttgttcct gcagtgcccg aaacccacac tgcagcagat
     4381 cagccacatc gcccagcagc ttgggctcga gaaggatgtg gtccgagtgt ggttctgtaa
     4441 ccggcgccag aagggcaagc gatcaagcag cgactatgca caacgagagg attttgaggc
     4501 tgctgggtct cctttctcag ggggaccagt gtcctttcct ctggccccag ggccccattt
     4561 tggtacccca ggctatggga gccctcactt cactgcactg tactcctcgg tccctttccc
     4621 tgagggggaa gcctttcccc ctgtctctgt caccactctg ggctctccca tgcattcaaa
     4681 ctgaggtaag ggcgaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
     4741 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
     4801 tatgtactat ttaaactcga gcggccgcca gcacagtggt cgacgataaa ataaaagatt
     4861 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     4921 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     4981 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     5041 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     5101 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     5161 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     5221 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5281 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     5341 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     5401 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     5461 gtcagcgggg gtctttcaca tgcagcatgt atcaaaatta atttggtttt tttcttaagt
     5521 atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5821 cgctcaagtc agaggtggcg aaacccgaca ggacta
//
