LOCUS       sequence-57025-e        9947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, 
            Ellisman MH, Tsien RY, Lin MZ
  TITLE     Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates 
            in light and electron microscopy.
  JOURNAL   Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 
            2012 Oct 28.
  PUBMED    23103964
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3246"; 
            journalName: "Nat Neurosci"; date: "2012-11"; volume: "15"; issue: 
            "12"; pages: "1742-51"
COMMENT     Sequence Label: PSD95-TS:YFP
FEATURES             Location/Qualifiers
     source          1..9947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(3936..3938,3939..3941,3942..4400)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(3981..4002)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4242..4261)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5259..5285
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     5483..5504
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5547..5573
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6971..6990)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7036..7091
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7090..7109)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7450..7466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7450..7466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7463..7485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7474..7490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7498..7504,7505..7522,7523..7528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7543..7564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7623..7818
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7669..7804
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7824..7958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7861..7880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7915..7934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8026..8043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8197..8785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8277..8296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8956..9747,9748..9816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9579..9598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9817..9921)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccggact ctagcctagg
     1741 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct
     1801 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca
     1861 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gtgaacggaa
     1921 cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct
     1981 tcagcatcgc aggtggcacc gacaacccac acatcggtga cgacccatcc atctttatca
     2041 ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc aacgacagca
     2101 tcctgtttgt caatgaagtg gatgtccggg aggtgaccca ttcagctgca gtggaggccc
     2161 tcaaagaggc gggttccatc gttcgcctct acgtcatgcg ccggaaaccc ccagctgaga
     2221 agatcataga gatcaagctt atcaaagggc ctaaaggact tggcttcagc atcgcagggg
     2281 gcgttgggaa ccagcacatc cctggagata atagcatcta cgtaaccaag atcatcgaag
     2341 gaggcgctgc ccacaaggat ggcaggttgc agatcggaga caagatcctg gcggtcaaca
     2401 gtgtggggct agaggatgtc atgcatgagg acgccgtggc agccctgaag aacacatatg
     2461 acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc
     2521 ccccagacat cacaacctca tattctcagc acctggacaa tgagatcagt catagcagct
     2581 acttgggcac cgactacccc acagccatga cccccacttc ccctcggcgc tactcccccg
     2641 tggccaagga cctgctaggg gaggaagata ttccccggga accaaggcgg atcgtgatcc
     2701 atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggacggt gaaggcatct
     2761 tcatctcctt catccttgct gggggtccag ctgacctcag tggggagcta cggaaggggg
     2821 accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcatgaa caggctgcca
     2881 ttgccctgaa gaacgcgggt cagacggtca cgatcatcgc tcagtataaa ccagaagagt
     2941 atagccgatt cgaggccaag atccatgatc ttcgggaaca gcttatgaat agtagcctgg
     3001 gctcagggac tgcatctctg cgaagcaacc ccaagcgggg cttctatatc agggccctgt
     3061 ttgactacga caagaccaag gactgcggtt tcttgagcca ggccctgagc ttccactttg
     3121 gggatgtgct tcatgtaatt gacgccagcg acgaagagtg gtggcaagcg cggcgggtcc
     3181 actctgacag tgagaccgat gacattggct tcattcccag caaacggcgg gtcgagcgac
     3241 gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca cagggtcgag
     3301 aagactcggt tctgagctat gagacggtga cgcagatgga agtgcactac gctcgcccca
     3361 tcatcatcct tgggcctacc aaagaccgtg ccaacgatga tcttctctcc gagttccccg
     3421 acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat gagatagacg
     3481 gccgcgatta ccactttgtc tcctcccggg agaaaatgga gaaggacatt caggcgcaca
     3541 agttcattga ggctggccag tacaacagcc acctctacgg gaccagcgtc cagtctgtgc
     3601 gagaggtagc agagcagggg aagcactgca tccttgatgt ctcagccaat gccgtgcggc
     3661 ggctgcaggc ggcccacctg caccctatcg ccatcttcat ccgtccccgc tccctggaga
     3721 atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc ttcgacaggg
     3781 ccacgaagct ggagcaggag ttcacggagt gcttctcagc catcgtagag ggcgacagct
     3841 ttgaagagat ctatcacaaa gtgaaacgtg tcatcgaaga cctctcaggc ccctacatct
     3901 gggtcccagc ccgagagaga ctggagcccg ggcagatggt gagcaagggc gaggagctgt
     3961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4141 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4381 acaacgtcta tatcaccgcc gacaagcagt tcgaaaccgg gcccatggcc agcatgactg
     4441 gtggacagca aatggggtcg acggaggacg tggtgtgctg ccactcaatc tacggcaaga
     4501 agaagggtga tatcgacacc taccgataca taggctcttc cgggacaggc tgcgtggtca
     4561 tagtgggcag gatcgtcttg tccggatccg gcactagtgc gcccatcacg gcgtacgccc
     4621 agcagacgag aggcctccta gggtgtataa tcaccagcct gactggccgg gacaaaaacc
     4681 aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca
     4741 tcaatggggt atgctgggca gtctaccacg gggccggaac gaggaccatc gcatcaccca
     4801 agggtcctgt catccagatg tataccaatg tggaccaaga ccttgtgggc tggcccgctc
     4861 ctcaaggttc ccgctcattg acaccctgta cctgcggctc ctcggacctt tacctggtca
     4921 cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt
     4981 cgccccggcc catttcctac ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg
     5041 gacacgccgt gggcctattc agggccgcgg tgtgcacccg tggagtggct aaagcggtgg
     5101 actttatccc tgtggagaac ctagagacaa ccatgagatc cccggtgttc acggacaact
     5161 cctctccacc agcagtcacc ctgacgcacc caatcaccaa aatcgatagg gaggttctct
     5221 accaggagtt cgatgagatg gaagagtgct ctcagcacta tccctacgat gtgcccgatt
     5281 acgctggcgc gtctgcatgc cagaagaacg gcatcaaggc caacttcaag atccgccaca
     5341 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5401 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
     5461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5521 ctctcggcat ggacgagctg tacaagtatc cctacgatgt gcccgattac gctggcgcgc
     5581 cttgattcct gccctggctt ggcctggact caccctgcct ccaccacctg ggcccttggt
     5641 ctggactgaa tagcccaagc ccttcgtacc ctagcctttc ttcccaccct tcttatttat
     5701 ttcctttctg actggatcga gcccattgga gggaggacac tcctctctgc atgtatccct
     5761 gcaccccaga tcagggcccc cagaccccag gaacctgggg tctggggggg agctgggctc
     5821 ctggttccga gcccttgctc cttaagactc ctacccctgc ccgcccccat gcacacacag
     5881 acccactggg ggcctctgcc ctccccattt tctcccacac acattccaga agtcagggcc
     5941 cctctcaagg agtacccgct gtagggatgc agggccacag gcttccgcgc tctcccgagg
     6001 caggggctgg ggtcacccct gccccatcat aactccccac gccgtttgag ttctccttta
     6061 ttttctccat tcttttcttc tcaaaggtgg tttttggggg gagaagcagg ggggctctcc
     6121 tgagggtccc cccgtctttc acacacctcc cacctttttt ctttgccggt ttgcatgagt
     6181 ggaaggtcta actgtggctt tttttttttt ctgggatttt atttcatttt atttgatttt
     6241 atttttttgg gaaaagggga gggatgggtc tggggagtgg ggaatgtggg aggggggtgg
     6301 gggggcaaag ggtcaggggt tgggtgtccg ggagccaggg gaggacagga aatgctgccg
     6361 ccttctgcaa tttatttatt tttttctttt gagagagtga aaggaagaga cagacacttg
     6421 aaaccccgtg tgtggcctgg ttctttgggg tgagggaggg aagtggcctg ggatgggtgg
     6481 ctctgagaac ttcagatcct tccttacctc acagagtata gggtgcctaa aggggtcatg
     6541 ggaaggactt accagaccga ggcagacaga gctgccaccg ccgggcctgg gaagagccgg
     6601 agctgggtta gcccctggca gactcgccat tgatccctgc aagctcagtc gagccctgac
     6661 cactccccct agctggcctc atccctggtg acagcacatt catccctaaa gagaaataca
     6721 gaggctcctc ggcttacagt ggagtgaaga ggacctatgt ataaaactac ccaggatgta
     6781 caaattcaat acacctaacc ggccaaacat cagggctcgg ctccacccat ccttaaaggc
     6841 tcagcacaca ggcccgcggt tgggcaaagc ctgttgtgtc acaaagcact gaacagctcg
     6901 tgtgacggcc tgggagctga cgctactgct gctcagcatc aagtgccacc caatgcggcc
     6961 gctcgacgga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     7021 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     7081 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     7141 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta
     7201 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     7261 gccttgactt gaggttagat ttttttttat attttgtttg tgttattttt ttctttaaca
     7321 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     7381 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc
     7441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     7621 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7801 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     7861 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7921 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     7981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
