LOCUS       sequence-57026-e       10286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, 
            Ellisman MH, Tsien RY, Lin MZ
  TITLE     Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates 
            in light and electron microscopy.
  JOURNAL   Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 
            2012 Oct 28.
  PUBMED    23103964
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3246"; 
            journalName: "Nat Neurosci"; date: "2012-11"; volume: "15"; issue: 
            "12"; pages: "1742-51"
COMMENT     Sequence Label: PSD95-TS:YSOG1
FEATURES             Location/Qualifiers
     source          1..10286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(3936..3938,3939..3941,3942..4400)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(3981..4002)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4242..4261)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5226..5543
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     CDS             5625..5651
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     5849..5870
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7310..7329)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7375..7430
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7429..7448)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7789..7805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7789..7805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7802..7824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7813..7829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7837..7843,7844..7861,7862..7867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7882..7903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7962..8157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8008..8143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8163..8297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8200..8219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8254..8273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8365..8382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8536..9124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8616..8635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9295..10086,10087..10155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9918..9937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10156..10260)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccggact ctagcctagg
     1741 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct
     1801 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca
     1861 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gtgaacggaa
     1921 cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct
     1981 tcagcatcgc aggtggcacc gacaacccac acatcggtga cgacccatcc atctttatca
     2041 ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc aacgacagca
     2101 tcctgtttgt caatgaagtg gatgtccggg aggtgaccca ttcagctgca gtggaggccc
     2161 tcaaagaggc gggttccatc gttcgcctct acgtcatgcg ccggaaaccc ccagctgaga
     2221 agatcataga gatcaagctt atcaaagggc ctaaaggact tggcttcagc atcgcagggg
     2281 gcgttgggaa ccagcacatc cctggagata atagcatcta cgtaaccaag atcatcgaag
     2341 gaggcgctgc ccacaaggat ggcaggttgc agatcggaga caagatcctg gcggtcaaca
     2401 gtgtggggct agaggatgtc atgcatgagg acgccgtggc agccctgaag aacacatatg
     2461 acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc
     2521 ccccagacat cacaacctca tattctcagc acctggacaa tgagatcagt catagcagct
     2581 acttgggcac cgactacccc acagccatga cccccacttc ccctcggcgc tactcccccg
     2641 tggccaagga cctgctaggg gaggaagata ttccccggga accaaggcgg atcgtgatcc
     2701 atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggacggt gaaggcatct
     2761 tcatctcctt catccttgct gggggtccag ctgacctcag tggggagcta cggaaggggg
     2821 accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcatgaa caggctgcca
     2881 ttgccctgaa gaacgcgggt cagacggtca cgatcatcgc tcagtataaa ccagaagagt
     2941 atagccgatt cgaggccaag atccatgatc ttcgggaaca gcttatgaat agtagcctgg
     3001 gctcagggac tgcatctctg cgaagcaacc ccaagcgggg cttctatatc agggccctgt
     3061 ttgactacga caagaccaag gactgcggtt tcttgagcca ggccctgagc ttccactttg
     3121 gggatgtgct tcatgtaatt gacgccagcg acgaagagtg gtggcaagcg cggcgggtcc
     3181 actctgacag tgagaccgat gacattggct tcattcccag caaacggcgg gtcgagcgac
     3241 gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca cagggtcgag
     3301 aagactcggt tctgagctat gagacggtga cgcagatgga agtgcactac gctcgcccca
     3361 tcatcatcct tgggcctacc aaagaccgtg ccaacgatga tcttctctcc gagttccccg
     3421 acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat gagatagacg
     3481 gccgcgatta ccactttgtc tcctcccggg agaaaatgga gaaggacatt caggcgcaca
     3541 agttcattga ggctggccag tacaacagcc acctctacgg gaccagcgtc cagtctgtgc
     3601 gagaggtagc agagcagggg aagcactgca tccttgatgt ctcagccaat gccgtgcggc
     3661 ggctgcaggc ggcccacctg caccctatcg ccatcttcat ccgtccccgc tccctggaga
     3721 atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc ttcgacaggg
     3781 ccacgaagct ggagcaggag ttcacggagt gcttctcagc catcgtagag ggcgacagct
     3841 ttgaagagat ctatcacaaa gtgaaacgtg tcatcgaaga cctctcaggc ccctacatct
     3901 gggtcccagc ccgagagaga ctggagcccg ggcagatggt gagcaagggc gaggagctgt
     3961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4141 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4381 acaacgtcta tatcaccgcc gacaagcagt tcgaaaccgg gcccatggcc agcatgactg
     4441 gtggacagca aatggggtcg acggaggacg tggtgtgctg ccactcaatc tacggcaaga
     4501 agaagggtga tatcgacacc taccgataca taggctcttc cgggacaggc tgcgtggtca
     4561 tagtgggcag gatcgtcttg tccggatccg gcactagtgc gcccatcacg gcgtacgccc
     4621 agcagacgag aggcctccta gggtgtataa tcaccagcct gactggccgg gacaaaaacc
     4681 aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca
     4741 tcaatggggt atgctgggca gtctaccacg gggccggaac gaggaccatc gcatcaccca
     4801 agggtcctgt catccagatg tataccaatg tggaccaaga ccttgtgggc tggcccgctc
     4861 ctcaaggttc ccgctcattg acaccctgta cctgcggctc ctcggacctt tacctggtca
     4921 cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt
     4981 cgccccggcc catttcctac ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg
     5041 gacacgccgt gggcctattc agggccgcgg tgtgcacccg tggagtggct aaagcggtgg
     5101 actttatccc tgtggagaac ctagagacaa ccatgagatc cccggtgttc acggacaact
     5161 cctctccacc agcagtcacc ctgacgcacc cagcggtccg cggtggcggc agtggtggcg
     5221 gcagcatgga gaaaagtttc gtgataactg atccacggct gccagacaat cccatcatct
     5281 tcgcatccga tggcttcctg gagctgaccg agtattccag agaggagatc ctgggccgca
     5341 atggccgctt tctgcaggga ccagagacag accaggccac agtgcagaag attcgcgatg
     5401 ccattagaga tcagcgcgag attaccgtgc agctgataaa ctacacaaaa agcgggaaga
     5461 aattctggaa cctcctgcac ctccagccca tgagggacca gaagggtgag ctccagtatt
     5521 tcatcggagt gcagctggat ggatcaggag gaggctcagg aggaggaatc gatagggagg
     5581 ttctctacca ggagttcgat gagatggaag agtgctctca gcactatccc tacgatgtgc
     5641 ccgattacgc tggcgcgtct gcatgccaga agaacggcat caaggccaac ttcaagatcc
     5701 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5761 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
     5821 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5881 ggatcactct cggcatggac gagctgtaca agggcgcgcc ttgattcctg ccctggcttg
     5941 gcctggactc accctgcctc caccacctgg gcccttggtc tggactgaat agcccaagcc
     6001 cttcgtaccc tagcctttct tcccaccctt cttatttatt tcctttctga ctggatcgag
     6061 cccattggag ggaggacact cctctctgca tgtatccctg caccccagat cagggccccc
     6121 agaccccagg aacctggggt ctggggggga gctgggctcc tggttccgag cccttgctcc
     6181 ttaagactcc tacccctgcc cgcccccatg cacacacaga cccactgggg gcctctgccc
     6241 tccccatttt ctcccacaca cattccagaa gtcagggccc ctctcaagga gtacccgctg
     6301 tagggatgca gggccacagg cttccgcgct ctcccgaggc aggggctggg gtcacccctg
     6361 ccccatcata actccccacg ccgtttgagt tctcctttat tttctccatt cttttcttct
     6421 caaaggtggt ttttgggggg agaagcaggg gggctctcct gagggtcccc ccgtctttca
     6481 cacacctccc accttttttc tttgccggtt tgcatgagtg gaaggtctaa ctgtggcttt
     6541 tttttttttc tgggatttta tttcatttta tttgatttta tttttttggg aaaaggggag
     6601 ggatgggtct ggggagtggg gaatgtggga ggggggtggg ggggcaaagg gtcaggggtt
     6661 gggtgtccgg gagccagggg aggacaggaa atgctgccgc cttctgcaat ttatttattt
     6721 ttttcttttg agagagtgaa aggaagagac agacacttga aaccccgtgt gtggcctggt
     6781 tctttggggt gagggaggga agtggcctgg gatgggtggc tctgagaact tcagatcctt
     6841 ccttacctca cagagtatag ggtgcctaaa ggggtcatgg gaaggactta ccagaccgag
     6901 gcagacagag ctgccaccgc cgggcctggg aagagccgga gctgggttag cccctggcag
     6961 actcgccatt gatccctgca agctcagtcg agccctgacc actcccccta gctggcctca
     7021 tccctggtga cagcacattc atccctaaag agaaatacag aggctcctcg gcttacagtg
     7081 gagtgaagag gacctatgta taaaactacc caggatgtac aaattcaata cacctaaccg
     7141 gccaaacatc agggctcggc tccacccatc cttaaaggct cagcacacag gcccgcggtt
     7201 gggcaaagcc tgttgtgtca caaagcactg aacagctcgt gtgacggcct gggagctgac
     7261 gctactgctg ctcagcatca agtgccaccc aatgcggccg ctcgacggat ctttttccct
     7321 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7381 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7441 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7501 catatgccat atgctggctg ccatgaacaa aggtggctat aaagaggtca tcagtatatg
     7561 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     7621 tttttttata ttttgtttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     7681 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     7741 ctcttatgaa gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     7801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7921 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     7981 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8041 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8101 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     8161 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8281 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8701 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9601 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9901 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10261 cacatttccc cgaaaagtgc cacctg
//
