LOCUS       sequence-57027-e       10289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Butko MT, Yang J, Geng Y, Kim HJ, Jeon NL, Shu X, Mackey MR, 
            Ellisman MH, Tsien RY, Lin MZ
  TITLE     Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates 
            in light and electron microscopy.
  JOURNAL   Nat Neurosci. 2012 Nov;15(12):1742-51. doi: 10.1038/nn.3246. Epub 
            2012 Oct 28.
  PUBMED    23103964
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3246"; 
            journalName: "Nat Neurosci"; date: "2012-11"; volume: "15"; issue: 
            "12"; pages: "1742-51"
COMMENT     Sequence Label: PSD95-TS:YSOG2
FEATURES             Location/Qualifiers
     source          1..10289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(3936..3938,3939..3941,3942..4400)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(3981..4002)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4242..4261)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             5256..5282
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     5480..5501
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5574..5891
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(7313..7332)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7378..7433
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7432..7451)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7792..7808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7792..7808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7805..7827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7816..7832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7840..7846,7847..7864,7865..7870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7885..7906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7965..8160
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8011..8146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8166..8300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8203..8222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8257..8276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8368..8385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8539..9127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8619..8638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9298..10089,10090..10158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9921..9940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10159..10263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccggact ctagcctagg
     1741 ccgcggccag gccccccaac atggactgtc tctgtatagt gacaaccaag aaataccgct
     1801 accaagatga agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca
     1861 attctccccc tgtgattgtc aacacggaca ccctagaagc cccaggatat gtgaacggaa
     1921 cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca ggtctgggct
     1981 tcagcatcgc aggtggcacc gacaacccac acatcggtga cgacccatcc atctttatca
     2041 ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc aacgacagca
     2101 tcctgtttgt caatgaagtg gatgtccggg aggtgaccca ttcagctgca gtggaggccc
     2161 tcaaagaggc gggttccatc gttcgcctct acgtcatgcg ccggaaaccc ccagctgaga
     2221 agatcataga gatcaagctt atcaaagggc ctaaaggact tggcttcagc atcgcagggg
     2281 gcgttgggaa ccagcacatc cctggagata atagcatcta cgtaaccaag atcatcgaag
     2341 gaggcgctgc ccacaaggat ggcaggttgc agatcggaga caagatcctg gcggtcaaca
     2401 gtgtggggct agaggatgtc atgcatgagg acgccgtggc agccctgaag aacacatatg
     2461 acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac agctatgctc
     2521 ccccagacat cacaacctca tattctcagc acctggacaa tgagatcagt catagcagct
     2581 acttgggcac cgactacccc acagccatga cccccacttc ccctcggcgc tactcccccg
     2641 tggccaagga cctgctaggg gaggaagata ttccccggga accaaggcgg atcgtgatcc
     2701 atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggacggt gaaggcatct
     2761 tcatctcctt catccttgct gggggtccag ctgacctcag tggggagcta cggaaggggg
     2821 accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcatgaa caggctgcca
     2881 ttgccctgaa gaacgcgggt cagacggtca cgatcatcgc tcagtataaa ccagaagagt
     2941 atagccgatt cgaggccaag atccatgatc ttcgggaaca gcttatgaat agtagcctgg
     3001 gctcagggac tgcatctctg cgaagcaacc ccaagcgggg cttctatatc agggccctgt
     3061 ttgactacga caagaccaag gactgcggtt tcttgagcca ggccctgagc ttccactttg
     3121 gggatgtgct tcatgtaatt gacgccagcg acgaagagtg gtggcaagcg cggcgggtcc
     3181 actctgacag tgagaccgat gacattggct tcattcccag caaacggcgg gtcgagcgac
     3241 gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca cagggtcgag
     3301 aagactcggt tctgagctat gagacggtga cgcagatgga agtgcactac gctcgcccca
     3361 tcatcatcct tgggcctacc aaagaccgtg ccaacgatga tcttctctcc gagttccccg
     3421 acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat gagatagacg
     3481 gccgcgatta ccactttgtc tcctcccggg agaaaatgga gaaggacatt caggcgcaca
     3541 agttcattga ggctggccag tacaacagcc acctctacgg gaccagcgtc cagtctgtgc
     3601 gagaggtagc agagcagggg aagcactgca tccttgatgt ctcagccaat gccgtgcggc
     3661 ggctgcaggc ggcccacctg caccctatcg ccatcttcat ccgtccccgc tccctggaga
     3721 atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc ttcgacaggg
     3781 ccacgaagct ggagcaggag ttcacggagt gcttctcagc catcgtagag ggcgacagct
     3841 ttgaagagat ctatcacaaa gtgaaacgtg tcatcgaaga cctctcaggc ccctacatct
     3901 gggtcccagc ccgagagaga ctggagcccg ggcagatggt gagcaagggc gaggagctgt
     3961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4141 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4381 acaacgtcta tatcaccgcc gacaagcagt tcgaaaccgg gcccatggcc agcatgactg
     4441 gtggacagca aatggggtcg acggaggacg tggtgtgctg ccactcaatc tacggcaaga
     4501 agaagggtga tatcgacacc taccgataca taggctcttc cgggacaggc tgcgtggtca
     4561 tagtgggcag gatcgtcttg tccggatccg gcactagtgc gcccatcacg gcgtacgccc
     4621 agcagacgag aggcctccta gggtgtataa tcaccagcct gactggccgg gacaaaaacc
     4681 aagtggaggg tgaggtccag atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca
     4741 tcaatggggt atgctgggca gtctaccacg gggccggaac gaggaccatc gcatcaccca
     4801 agggtcctgt catccagatg tataccaatg tggaccaaga ccttgtgggc tggcccgctc
     4861 ctcaaggttc ccgctcattg acaccctgta cctgcggctc ctcggacctt tacctggtca
     4921 cgaggcacgc cgatgtcatt cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt
     4981 cgccccggcc catttcctac ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg
     5041 gacacgccgt gggcctattc agggccgcgg tgtgcacccg tggagtggct aaagcggtgg
     5101 actttatccc tgtggagaac ctagagacaa ccatgagatc cccggtgttc acggacaact
     5161 cctctccacc agcagtcacc ctgacgcacc caatcaccaa aatcgatgag gacgtggtgt
     5221 gctgccactc aatctacggc aagaagaaga ccggttatcc ctacgatgtg cccgattacg
     5281 ctggcgcgtc tgcatgccag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     5341 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     5401 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     5461 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     5521 tcggcatgga cgagctgtac aagcggtccg gtggcggcag tggtggcggc agcatggaga
     5581 aaagtttcgt gataactgat ccacggctgc cagacaatcc catcatcttc gcatccgatg
     5641 gcttcctgga gctgaccgag tattccagag aggagatcct gggccgcaat ggccgctttc
     5701 tgcagggacc agagacagac caggccacag tgcagaagat tcgcgatgcc attagagatc
     5761 agcgcgagat taccgtgcag ctgataaact acacaaaaag cgggaagaaa ttctggaacc
     5821 tcctgcacct ccagcccatg agggaccaga agggtgagct ccagtatttc atcggagtgc
     5881 agctggatgg atcaggagga ggctcaggag gaggaggcgc gccttgattc ctgccctggc
     5941 ttggcctgga ctcaccctgc ctccaccacc tgggcccttg gtctggactg aatagcccaa
     6001 gcccttcgta ccctagcctt tcttcccacc cttcttattt atttcctttc tgactggatc
     6061 gagcccattg gagggaggac actcctctct gcatgtatcc ctgcacccca gatcagggcc
     6121 cccagacccc aggaacctgg ggtctggggg ggagctgggc tcctggttcc gagcccttgc
     6181 tccttaagac tcctacccct gcccgccccc atgcacacac agacccactg ggggcctctg
     6241 ccctccccat tttctcccac acacattcca gaagtcaggg cccctctcaa ggagtacccg
     6301 ctgtagggat gcagggccac aggcttccgc gctctcccga ggcaggggct ggggtcaccc
     6361 ctgccccatc ataactcccc acgccgtttg agttctcctt tattttctcc attcttttct
     6421 tctcaaaggt ggtttttggg gggagaagca ggggggctct cctgagggtc cccccgtctt
     6481 tcacacacct cccacctttt ttctttgccg gtttgcatga gtggaaggtc taactgtggc
     6541 tttttttttt ttctgggatt ttatttcatt ttatttgatt ttattttttt gggaaaaggg
     6601 gagggatggg tctggggagt ggggaatgtg ggaggggggt gggggggcaa agggtcaggg
     6661 gttgggtgtc cgggagccag gggaggacag gaaatgctgc cgccttctgc aatttattta
     6721 tttttttctt ttgagagagt gaaaggaaga gacagacact tgaaaccccg tgtgtggcct
     6781 ggttctttgg ggtgagggag ggaagtggcc tgggatgggt ggctctgaga acttcagatc
     6841 cttccttacc tcacagagta tagggtgcct aaaggggtca tgggaaggac ttaccagacc
     6901 gaggcagaca gagctgccac cgccgggcct gggaagagcc ggagctgggt tagcccctgg
     6961 cagactcgcc attgatccct gcaagctcag tcgagccctg accactcccc ctagctggcc
     7021 tcatccctgg tgacagcaca ttcatcccta aagagaaata cagaggctcc tcggcttaca
     7081 gtggagtgaa gaggacctat gtataaaact acccaggatg tacaaattca atacacctaa
     7141 ccggccaaac atcagggctc ggctccaccc atccttaaag gctcagcaca caggcccgcg
     7201 gttgggcaaa gcctgttgtg tcacaaagca ctgaacagct cgtgtgacgg cctgggagct
     7261 gacgctactg ctgctcagca tcaagtgcca cccaatgcgg ccgctcgacg gatctttttc
     7321 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     7381 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     7441 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     7501 caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat
     7561 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     7621 attttttttt atattttgtt tgtgttattt ttttctttaa catccctaaa attttcctta
     7681 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     7741 cttctcttat gaagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     7801 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7861 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7921 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     7981 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8041 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8101 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8161 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8281 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8701 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     8761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8941 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10261 gcgcacattt ccccgaaaag tgccacctg
//
