LOCUS       sequence-57032-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Maitra M, Cano CA, Garcia CK
  TITLE     Mutant surfactant A2 proteins associated with familial pulmonary 
            fibrosis and lung cancer induce TGF-beta1 secretion.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21064-9. doi: 
            10.1073/pnas.1217069110. Epub 2012 Dec 5.
  PUBMED    23223528
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217069110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-18- 18"; volume: "109"; issue: "51"; pages: "21064-9"
COMMENT     Sequence Label: pLenti-SPA2G231V
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             191..232
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agctntctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 cgtcgacatg tggctgtgcc ctctggccct caccctcatc ttgatggcag cctctggtgc
      181 tgcgtgcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggtgaagga
      241 cgtttgtgtt ggaagccctg gtatccccgg cactcctgga tcccacggcc tgccaggcag
      301 ggacgggaga gatggtgtca aaggagaccc tggccctcca ggccccatgg gtccgcctgg
      361 agaaacacca tgtcctcctg ggaataatgg gctgcctgga gcccctggtg tccctggaga
      421 gcgtggagag aagggggagg ctggcgagag aggccctcca gggcttccag ctcatctaga
      481 tgaggagctc caagccacac tccacgactt cagacatcaa atcctgcaga caaggggagc
      541 cctcagtctg cagggctcca taatgacagt aggagagaag gtcttctcca gcaatgggca
      601 gtccatcact tttgatgcca ttcaggaggc atgtgccaga gcaggcggcc gcattgctgt
      661 cccaaggaat ccagaggaaa atgaggccat tgcaagcttc gtgaagaagt acaacacata
      721 tgcctatgta ggcctgactg agggtcccag ccctggagac ttccgctact cagatgggac
      781 ccctgtaaac tacaccaact ggtaccgagg ggagcctgca ggtcggggaa aagagcagtg
      841 tgtggagatg tacacagatg tgcagtggaa tgacaggaac tgcctgtact cccgactgac
      901 catctgtgag ttctgaacta
//
