LOCUS       sequence-57068-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Papageorgis P, Lambert AW, Ozturk S, Gao F, Pan H, Manne U, 
            Alekseyev YO, Thiagalingam A, Abdolmaleky HM, Lenburg M, 
            Thiagalingam S
  TITLE     Smad signaling is required to maintain epigenetic silencing during 
            breast cancer progression.
  JOURNAL   Cancer Res. 2010 Feb 1;70(3):968-78. Epub 2010 Jan 19.
  PUBMED    20086175
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2010-02-1"; volume: "70(3)"; pages: "968-78. Epub 2010
            Jan 19"
COMMENT     Sequence Label: pLKO-shSmad2
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        290..799
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     719..737
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tatatcttgt ggaaaggacg aaacaccggg cctgatcttc acagtcatca ctcgagtgat
       61 gactgtgaag atcaggcttt ttgaattctc gacctcgaga caaatggcag tattcatcca
      121 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      241 tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt
      301 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
      361 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
      421 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
      481 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
      541 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
      601 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
      661 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
      721 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
      781 tcaccgacct ctctcccca
//
