LOCUS       sequence-57080-e        3811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   von Gise A, Lin Z, Schlegelmilch K, Honor LB, Pan GM, Buck JN, Ma Q,
            Ishiwata T, Zhou B, Camargo FD, Pu WT
  TITLE     YAP1, the nuclear target of Hippo signaling, stimulates heart growth
            through cardiomyocyte proliferation but not hypertrophy.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2394-9. doi: 
            10.1073/pnas.1116136109. Epub 2012 Jan 30.
  PUBMED    22308401
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1116136109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-02-14- 14"; volume: "109"; issue: "7"; pages: "2394-9"
COMMENT     Sequence Label: pENTR-3xFLAG-YAP1-S127A
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             489..560
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    complement(2040..2139)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2251..2270)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2262..3071
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2329..2348)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3161..3749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3650..3669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg
      481 acaccatgga ctacaaggac gacgatgaca aggattacaa agatgacgac gataaggact
      541 ataaggacga tgatgataaa ctggtaccta tggatcccgg gcagcagccg ccgcctcaac
      601 cggcccccca gggccaaggg cagccgcctt cgcagccccc gcaggggcag ggcccgccgt
      661 ccggacccgg gcaaccggca cccgcggcga cccaggcggc gccgcaggca ccccccgccg
      721 ggcatcagat cgtgcacgtc cgcggggact cggagaccga cctggaggcg ctcttcaacg
      781 ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt gcccatgagg ctccggaagc
      841 tgcccgactc cttcttcaag ccgccggagc ccaaatccca ctcccgacag gccagtactg
      901 atgcaggcac tgcaggagcc ctgactccac agcatgttcg agctcatgcc tctccagctt
      961 ctctgcagtt gggagctgtt tctcctggga cactgacccc cactggagta gtctctggcc
     1021 cagcagctac acccacagct cagcatcttc gacagtcttc ttttgagata cctgatgatg
     1081 tacctctgcc agcaggttgg gagatggcaa agacatcttc tggtcagaga tacttcttaa
     1141 atcacatcga tcagacaaca acatggcagg accccaggaa ggccatgctg tcccagatga
     1201 acgtcacagc ccccaccagt ccaccagtgc agcagaatat gatgaactcg gcttcaggtc
     1261 ctcttcctga tggatgggaa caagccatga ctcaggatgg agaaatttac tatataaacc
     1321 ataagaacaa gaccacctct tggctagacc caaggcttga ccctcgtttt gccatgaacc
     1381 agagaatcag tcagagtgct ccagtgaaac agccaccacc cctggctccc cagagcccac
     1441 agggaggcgt catgggtggc agcaactcca accagcagca acagatgcga ctgcagcaac
     1501 tgcagatgga gaaggagagg ctgcggctga aacagcaaga actgcttcgg caggagttag
     1561 ccctgcgtag ccagttacca acactggagc aggatggtgg gactcaaaat ccagtgtctt
     1621 ctcccgggat gtctcaggaa ttgagaacaa tgacgaccaa tagctcagat cctttcctta
     1681 acagtggcac ctatcactct cgagatgaga gtacagacag tggactaagc atgagcagct
     1741 acagtgtccc tcgaacccca gatgacttcc tgaacagtgt ggatgagatg gatacaggtg
     1801 atactatcaa ccaaagcacc ctgccctcac agcagaaccg tttcccagac taccttgaag
     1861 ccattcctgg gacaaatgtg gaccttggaa cactggaagg agatggaatg aacatagaag
     1921 gagaggagct gatgccaagt ctgcaggaag ctttgagttc tgacatcctt aatgacatgg
     1981 agtctgtttt ggctgccacc aagctagata aagaaagctt tcttacatgg ttattctaga
     2041 cccagctttc ttgtacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     2101 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
     2161 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2221 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2281 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2341 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2401 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2461 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2521 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2581 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2641 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2701 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2761 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2821 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2881 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2941 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3001 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3061 agtttttcta atcagaattg gttaattggt tgtaacatta ttcagattgg gccccgttcc
     3121 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3301 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3541 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3781 tggccttttg ctggcctttt gctcacatgt t
//
