LOCUS       sequence-57090-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 nuclease for genome 
            engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
      121 tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca
      181 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
      241 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
      301 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
      361 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
      421 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
      481 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
      541 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
      601 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
      661 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
      721 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
      781 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
      841 ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag
      901 atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc
      961 agatcggcga ccagtacgca gacctttttt tggcgg
//
