LOCUS       sequence-57092-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 D10A mutant which functions as 
            a nickase for genome engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9_D10A
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      118..127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcg aacccttgcc
      121 accatggaca agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc
      181 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat
      241 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc
      301 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc
      361 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat
      421 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt
      481 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg
      541 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg
      601 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc
      661 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac
      721 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc
      781 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt
      841 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc
      901 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg
      961 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcaga
//
