LOCUS       sequence-57093-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon optimized Cas9 D10A mutant which functions as 
            a nickase for genome engineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE,
            Church GM
  TITLE     RNA-Guided Human Genome Engineering via Cas9.
  JOURNAL   Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033
  PUBMED    23287722
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-02-15- 15"; volume: "339"; 
            issue: "6121"; pages: "823-6"
COMMENT     Sequence Label: hCas9_D10A
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..56)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actcattact aaccggtagg gatcgaaccc tttcacacct tcctcttctt cttggggtca
       61 gccctgctgt ctccaccgag ctgagagagg tcgattcttg tttcatagag ccccgtaatt
      121 gactgatgaa tcagtgtggc gtccaggacc tcctttgtag aggtgtaccg ctttctgtct
      181 atggtggtgt cgaagtactt gaaggctgca ggcgcgccca agttggtcag agtaaacaag
      241 tggataatgt tttctgcctg ctccctgatg ggcttatccc tgtgcttatt gtaagcagaa
      301 agcaccttat cgaggttagc gtcggcgagg atcactcttt tggagaattc gcttatttgc
      361 tcgatgatct catcaaggta gtgtttgtgt tgttccacga acagctgctt ctgctcatta
      421 tcttcgggag accctttgag cttttcatag tggctggcca gatacaagaa attaacgtat
      481 ttagagggca gtgccagctc gttacctttc tgcagctcgc ccgcactagc gagcattcgt
      541 ttccggccgt tttcaagctc aaagagagag tacttgggaa gcttaatgat gaggtctttt
      601 ttgacctctt tatatccttt cgcctcgaga aagtcgatgg ggtttttttc gaagcttgat
      661 cgctccatga ttgtgatgcc cagcagttcc ttgacgcttt tgagtttttt agacttccct
      721 ttctccactt tggccacaac cagtacactg taagcgactg taggagaatc gaatccgccg
      781 tatttcttgg ggtcccaatc ttttttgcgt gcgatcagct tgtcgctgtt ccttttcggg
      841 aggatacttt ccttggagaa gcctccggtc tgtacttcgg tctttttaac gatgttcacc
      901 tgcggcatgg acaggacctt ccggactgtc gcgaaatccc tacccttgtc ccacacgatt
      961 tctcctgttt ctccgtttgt ttcga
//
