LOCUS       sequence-57107-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD
  TITLE     Isolating and engineering human antibodies using yeast surface 
            display.
  JOURNAL   Nat Protoc. 2006;1(2):755-68.
  PUBMED    17406305
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(2)"; pages: "755-68"
COMMENT     Sequence Label: pCTcon2
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(351..380)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(633..659)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(660..671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atacgactca ctatagggcg aattggagct caattctctt aggattcgat tcacattcat
       61 ctttttttag ctactaatag gataaattat aggaatttat aacaaattga aatatggcag
      121 gcagcaaaat taaaattgtc ttagtttttt agtgtataga agtgaatagc tatataaagt
      181 atgtgtaaag ttggtaacgg aacgaaaaat agaaaaggat attacatggg aaaacatgtt
      241 gtttacggag aaatgaaaag tatattgtat tttgtacgag ctaaaagtac agtgggaaca
      301 aagtcgattt tgttacatct acactgttgt tatcagatct cgagctatta caagtcctct
      361 tcagaaataa gcttttgttc ggatccgccc ccgcagtcga cctgggaaga ttgaatagaa
      421 taacaatatt gagtagatgg agaatttttt tcagatggat tagctggttc acaattgtaa
      481 atattaatat atggagtatg agctctaata aaattcaaag attccatttt caaaaaatga
      541 gaaattttgc tagtaccgcc gccaccgcag ctagccgacc ctccgcctcc gctaccgcct
      601 ccaccagagc ctcctccacc actagcctgc agagcgtagt ctggaacgtc gtatgggtat
      661 ctaccttcaa tcgtcgagct attgtcctta aaaacatact gtgtgtttat ggggctgcct
      721 ttgctagttg ttgaggggtg agaaccgcaa ttactgacaa acgttactga tttgtaatat
      781 tcaaaaactc cttgcattgc cttcccgttg gccaaaatag tagtcgttga caaagagtac
      841 ggcgtcgatt ctaaagttgg tgaggggatt tgctcgcata tagttgtcag ttc
//
