LOCUS       sequence-57128-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt5A
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..54)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctaccggta cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa
       61 ccgcgggccc tctagatcaa ccactttgta caagaaagct gggtcggcgc gcccaccctt
      121 cagaattcac ttgcacacaa actggtccac gatctccgtg cacttcttgc acttgacgta
      181 gcagcaccag tggaacttgc agtggcagcg ctccgtctgc acggtcttga actggtcgta
      241 gccacggccg cagcacatga gctcgcagcc atccatgccc tccgacgtct tgttgcacag
      301 gcggccctgc gtgcccagcg agccggtgct ctcattgcgc acgcagtagt cagggctggg
      361 gtcgatgtag accaggtctt gtgtggtggg cgagttgaag cggctgttga cctgtaccaa
      421 cttgccccgg ctgttgagcc gcatggccgc cgcgctgtcg tacttctcct tcagggcatc
      481 acccaccttg cggaagtctg ccagctgcag ccagcatgtc ttcaggctac atgagccgga
      541 caccccatgg cacttgcagg ccacatcagc caggttgtac accgtcctgc ggccggcctc
      601 gttgttgtgc aggttcatga ggatgcgagc actctcgtag gagcccttgg cgtggatgcg
      661 ctcccgctcg cgggcgtcca cgaactcctt ggcaaagcgg tagccatagt cgatgttgtc
      721 gccgcagccg ccccagagcc agtcccgcgg caggtccttg gggcgcgcgg cgcggctgca
      781 gccgcaggtg gacagctcgc cctcgcggca cgcccggctc atggcgttca ccacccctgc
      841 tgcgctcacc gcgtatgtga aggccgtctc gcggctgcct atctgcatca ccctgccaaa
//
