LOCUS       sequence-57173-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPKCA
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgccttgggt ccagctttct ggacatgctg ggaacttcca ggcaggagag
      121 gatggtcgga ttctgaaacg tttctgtcag tgtgagcagc gcagcctgga gcagctgatg
      181 aaagacccgc tgcgaccttt cgtgcctgcc tactatggca tggtgctgca ggatggccag
      241 accttcaacc agatggaaga cctcctggct gactttgagg gcccctccat tatggactgc
      301 aagatgggca gcaggaccta tctggaagag gagctagtga aggcacggga acgtccccgt
      361 ccccggaagg acatgtatga gaagatggtg gctgtggacc ctggggcccc tacccctgag
      421 gagcatgccc agggtgcagt caccaagccc cgctacatgc agtggaggga aaccatgagc
      481 tccacctcta ccctgggctt ccggatcgag ggcatcaaga aggcagatgg gacctgtaac
      541 accaacttca agaagacgca ggcactggag caggtgacaa aagtgctgga ggacttcgtg
      601 gatggagacc acgtcatcct gcaaaagtac gtggcatgcc tagaagaact tcgtgaagct
      661 ctggagatct cccccttctt caagacccac gaggtggtag gcagctccct cctcttcgtg
      721 cacgaccaca ccggcctggc caaggtctgg atgatagact tcggcaagac ggtggccttg
      781 cccgaccacc agacgctcag ccacaggctg ccctgggctg agggcaaccg tgaggacggc
      841 tacctctggg gcctggacaa catgatctgc ctcctgcagg ggctggcaca gagctgataa
      901 cagtaaaggt ggatacggat ccgaa
//
