LOCUS       sequence-57176-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTPSA
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaagtacat tctggttact ggtggtgtta tatcaggaat tggaaaagga
      121 atcattgcca gcagtgtggg cacaatactc aagtcatgtg gtttacatgt aacttcaatc
      181 aaaattgacc cctacattaa cattgatgca ggaacattct ctccttatga gcatggtgag
      241 gtttttgtgc tggatgatgg tggggaagta gaccttgacc tgggtaacta tgagcggttc
      301 cttgacatcc gcctcaccaa ggacaataat ctgaccactg gaaagatata ccagtatgtc
      361 attaacaagg aacggaaagg agattacttg gggaaaactg tccaagttgt ccctcatatc
      421 acagatgcaa tccaggagtg ggtgatgaga caggcgttaa tacctgtaga tgaagatggc
      481 ctggaacctc aagtgtgtgt tattgagctt ggtggaaccg tgggggacat agaaagcatg
      541 ccctttattg aggccttccg tcagttccaa ttcaaggtca aaagagagaa cttttgtaac
      601 atccacgtca gtctagttcc ccagccaagt tcaacagggg aacagaagac taaacctacc
      661 cagaatagtg ttcgggaact tagaggactt gggctttccc cagatctggt tgtatgcagg
      721 tgctcaaatc cacttgacac atcagtgaag gagaaaatat caatgttctg ccatgttgag
      781 cctgaacaag tgatctgtgt ccacgatgtc tcatccatct accgagtccc cttgttgtta
      841 gaggagcaag gggttgtaga ttattttctt cgaagacttg accttcctat tgagaggcag
      901 ccaagaaaaa tgctgatgaa atggaaagag atggctgaca gatatgatcg ctaacagtaa
      961 aggtggatac ggatccgaa
//
