LOCUS       sequence-57178-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPAA
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggttccgcg tggtagtatg
       61 gcggcctcat tggtggggaa gaagatcgtg tttgtaacgg ggaacgccaa gaagctggag
      121 gaggtcgttc agattctagg agataagttt ccatgcactt tggtggcaca gaaaattgac
      181 ctgccggagt accaggggga gccggatgag atttccatac agaaatgtca ggaggcagtt
      241 cgccaggtac aggggcccgt gctggttgag gacacttgtc tgtgcttcaa tgcccttgga
      301 gggctccccg gcccctacat aaagtggttt ctggagaagt taaagcctga aggtctccac
      361 cagctcctgg ccgggttcga ggacaagtca gcctatgcgc tctgcacgtt tgcactcagc
      421 accggggacc caagccagcc cgtgcgcctg ttcaggggcc ggacctcggg ccggatcgtg
      481 gcacccagag gctgccagga ctttggctgg gacccctgct ttcagcctga tggatatgag
      541 cagacgtacg cagagatgcc taaggcggag aagaacgctg tctcccatcg cttccgggcc
      601 ctgctggagc tgcaggagta ctttggcagt ttggcagctt gatgacgaag cttg
//
